Multiple integrins share the ability to induce elevation of intracellular pH.
Multiple integrins share the ability to induce elevation of intracellular pH.
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DOI：
10.1016/0014-4827(91)90407-l
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发表时间：
1991-08
影响因子：
3.7
通讯作者：
Martin A. Schwartz;Donald E. Ingber;Michael B. Lawrence;T. A. Springer;Claude Lechene;Claude Lechene
中科院分区：
文献类型：
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作者：
Martin A. Schwartz;Donald E. Ingber;Michael B. Lawrence;T. A. Springer;Claude Lechene;Claude Lechene
先前的工作已经表明，贴壁依赖性细胞通过整合素α5β1粘附到纤连蛋白上，导致Na-H反向转运蛋白的激活和细胞内pH（pHi）的升高。我们现在表明，牛毛细血管内皮细胞（BCE）的粘附纤维蛋白原，胶原蛋白III型，IV型和V型，层粘连蛋白和玻连蛋白，配体结合其他成员的整合素家族，导致显着升高的pH值。其他配体（碱性成纤维细胞生长因子、伴刀豆球蛋白A和凝血酶）在固定于塑料上时结合细胞，但不结合整联蛋白且不支持细胞生长，不升高pHi。与抗整联蛋白αvβ3抗体的粘附也会升高pHi。粘附的外周血人T淋巴细胞的抗体对整合素LFA-1诱导的pHi上升。CD 2或ICAM-2的抗体对pHi只有轻微的影响，而强烈激活T细胞的T细胞受体复合物的抗体诱导了pHi的大幅增加。我们的结论是，由整合素升高的pHi是特定的，是整合素家族的许多成员所共有的属性。
Previous work has shown that adhesion of anchorage-dependent cells to fibronectin via integrin α5β1 leads to activation of the Na-H antiporter and a rise in intracellular pH (pHi). We now show that adhesion of bovine capillary endothelial cells (BCE) to fibrinogen; collagens type III, IV, and V; laminin; and vitronectin; ligands that bind other members of the integrin family, resulted in significant elevations in pHi. Other ligands (basic fibroblast growth factor, concanavalin A, and thrombin), which bind cells when immobilized on plastic, but that do not bind integrins and do not support cell growth, do not elevate pHi. Adhesion to an antibody against integrin αvβ3 also elevates pHi. Adhesion of peripheral human T lymphocytes to an antibody against the integrin LFA-1 induced a rise in pHi. Antibodies to CD2 or ICAM-2 had only slight effects on pHi, whereas an antibody to the T cell receptor complex that strongly activates T cells induced a large increase in pHi. We conclude that elevation of pHi by integrins is specific and is a property shared by many members of the integrin family.