A TgfßRI/Snai1-dependent developmental module at the core of vertebrate axial elongation
A TgfßRI/Snai1-dependent developmental module at the core of vertebrate axial elongation
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脊椎动物轴向伸长的核心依赖于 TgfäRI/Snai1 的发育模块
DOI：
10.1101/2020.03.09.983809
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发表时间：
2020
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影响因子：
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通讯作者：
Dias A
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作者：
Dias A
脊椎动物颅后体轴的形成遵循两个连续但不同的阶段。第一阶段通过上胚层中轴祖细胞上原条的活动产生骶骨前结构（所谓的初级体）。然后胚胎转变为产生次级体（后骶骨结构），这取决于尾芽中的轴向祖细胞。在这里，我们表明，哺乳动物的尾芽是通过一个独立的功能发育模块，并发，但功能不同，从产生的主要机构。这个模块是触发收敛Tgfbr 1和Snai1活动，促进一个不完整的上皮细胞向间充质转化的上胚层轴向祖细胞的一个子集。这种EMT在功能上不同于由原条协调的EMT，因为它不会导致中胚层分化，而是使轴向祖细胞进入过渡状态，保持其祖细胞活性以驱动进一步的轴向体延伸。
Formation of the vertebrate postcranial body axis follows two sequential but distinct phases. The first phase generates pre-sacral structures (the so-called primary body) through the activity of the primitive streak on axial progenitors within the epiblast. The embryo then switches to generate the secondary body (post-sacral structures), which depends on axial progenitors in the tail bud. Here we show that the mammalian tail bud is generated through an independent functional developmental module, concurrent but functionally different from that generating the primary body. This module is triggered by convergent Tgfbr1 and Snai1 activities that promote an incomplete epithelial to mesenchymal transition on a subset of epiblast axial progenitors. This EMT is functionally different from that coordinated by the primitive streak, as it does not lead to mesodermal differentiation but brings axial progenitors into a transitory state, keeping their progenitor activity to drive further axial body extension.