Relation of DNA ploidy to genetic aberrations detected by chromosomal CGH and FISH in gastric adenocarcinomas.
Relation of DNA ploidy to genetic aberrations detected by chromosomal CGH and FISH in gastric adenocarcinomas.
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DOI：
10.3892/or.15.6.1491
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发表时间：
2006-06
期刊：
影响因子：
4.2
通讯作者：
T. Furuya;T. Uchiyama;A. Adachi;Y. Chochi;A. Oga;S. Kawauchi;Kimio Ishiglo;K. Sasaki
中科院分区：
文献类型：
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作者：
T. Furuya;T. Uchiyama;A. Adachi;Y. Chochi;A. Oga;S. Kawauchi;Kimio Ishiglo;K. Sasaki
我们分析了DNA拷贝数畸变（DCNAs）的染色体比较基因组杂交（CGH）在93个连续的散发性胃腺癌。此外，通过荧光原位杂交（FISH）评价了7、11、17和18号染色体的数目畸变。根据激光扫描细胞仪（LSC）测定的细胞核DNA含量，将36例胃癌分为DNA二倍体（1.0或= 1.2）组。在二倍体和非整倍体肿瘤中，DNA拷贝数的增加和丢失最频繁，分别见于8 q21 -23和19p13.3。二倍体癌症根据遗传畸变进一步分为主要类型和亚型癌症。二倍体癌症组包括9种亚型癌症，显示大量DCNA; CGH检测到的DCNA平均数量为26.7个/肿瘤。在这些二倍体亚型癌症中，该值比二倍体主要类型癌症大得多（平均值，每个肿瘤5.2，p<0.0001）。这九种癌症的特征还在于染色体拷贝数的大细胞间变异，这在27种主要的二倍体类型癌症中没有检测到。非整倍体癌症组仅包括三种亚型肿瘤，仅显示少量DCNA（平均每个肿瘤3个）和染色体拷贝数的最小细胞间变异。这些数据表明，胃腺癌可以分为三种类型：非整倍体，主要二倍体型和二倍体亚型癌。大规模的研究是必要的，以澄清这些类型之间的生物学特性和潜在的遗传机制的差异。
We analyzed DNA copy number aberrations (DCNAs) by chromosomal comparative genomic hybridization (CGH) in 93 consecutive sporadic gastric adenocarcinomas. In addition, numerical aberrations in chromosomes 7, 11, 17, and 18 were evaluated by fluorescence in situ hybridization (FISH). Gastric cancers were divided on the basis of nuclear DNA content measured by laser scanning cytometry (LSC) into two groups, 36 DNA diploid (1.0 or= 1.2) cancers. The most frequent gain and loss of DNA copy number were found at 8q21-23 and 19p13.3, respectively, in both diploid and aneuploid cancers. Diploid cancers were further divided on the basis of genetic aberrations into major type and subtype cancers. The diploid cancer group included nine subtype cancers that showed large numbers of DCNAs; the mean number of DCNAs detected by CGH was 26.7 per tumor. This value was much larger in these diploid subtype cancers than diploid major type cancers (mean, 5.2 per tumor, p<0.0001). These nine cancers were also characterized by large intercellular variations in chromosome copy numbers that were not detected in the 27 major diploid type cancers. The aneuploid cancer group included only three subtype tumors that showed only a small number of DCNAs (mean, 3 per tumor) and minimal intercellular variations in chromosomal copy number. These data indicate that gastric adenocarcinomas can be divided into three types; aneuploid, major diploid type and diploid subtype cancers. Large-scale studies are necessary to clarify the differences in biological characteristics and underlying genetic mechanisms between these types.