Tissue-specific transcriptome for Rheum tanguticum reveals candidate genes related to the anthraquinones biosynthesis
Tissue-specific transcriptome for Rheum tanguticum reveals candidate genes related to the anthraquinones biosynthesis
复制标题
唐古特大黄的组织特异性转录组揭示了与蒽醌生物合成相关的候选基因
DOI：
10.1007/s12298-021-01099-8
复制
发表时间：
2021-11-17
影响因子：
3.5
通讯作者：
Wang，Xumei
中科院分区：
文献类型：
--
作者：
Zhou，Tao;Zhang，Tianyi;Wang，Xumei
Rheum tanguticum(Maxim. ex Regel) Maxim. ex Balf. is a herbaceous perennial plant indigenous to China, and its root and rhizomes were usually used as an important traditional Chinese medicine. However, the genomic resources are still scarce forR. tanguticumand even forRheumgenus. Transcriptome datasets from different tissues ofR. tanguticumwere obtained to screen the genes related to anthraquinones biosynthesis, and five free anthraquinones were also determined. Nine cDNA libraries of roots, stems and leaves were generated, and a total of 272 million high-quality reads were assembled into 257,942 unigenes. Based on the functional annotation, A total of 227 candidate enzyme genes involved in the MVA, MEP, shikimate and polyketide pathways were identified, and several differentially expressed genes found functionally associated with anthraquinones biosynthesis showed distinct tissue-specific expression patterns. Especially, we found that the expression levels ofPKSIII genes might result in the content differences of free anthraquinones in different tissues ofR. tanguticum. Besides, 137,400 SSR loci were identified, and 64,081 SSR primer pairs were successfully designed based on these loci. Our results not only provide cues for the genetic mechanism of anthraquinone content differences in different tissues ofR. tanguticum, but also lay genomic foundation for the subsequent genetic engineering and breeding forRheumspecies.Supplementary information:The online version contains supplementary material available at 10.1007/s12298-021-01099-8.
