Localization of zinc in the outer retina of carp: A light‐ and electron‐microscopic study
Localization of zinc in the outer retina of carp: A light‐ and electron‐microscopic study
复制标题
DOI：
10.1002/syn.20501
复制
发表时间：
2008-05
期刊：
影响因子：
2.3
通讯作者：
Shu-Chen Lee;Y. Zhong;Rui-Xi Li;Zhang Yu;Xiong-Li Yang
中科院分区：
文献类型：
--
作者：
Shu-Chen Lee;Y. Zhong;Rui-Xi Li;Zhang Yu;Xiong-Li Yang
许多证据表明，锌可能在包括视网膜在内的中枢神经系统中发挥重要的神经调节作用。本工作采用银放大法，在光镜和电镜下研究了锌在鲤鱼视网膜外层的定位。反应产物（银颗粒）广泛分布于整个视网膜，包括光感受器、外核层和内核层（ONL和INL）、神经节细胞层（GCL）以及外丛状层和内丛状层（OPL和IPL）。一般来说，锌的染色在视网膜外层比在视网膜内层更强，颗粒聚集在OPL和外界膜（OLM）的密度最大。银沉淀物也被检测到的内段，轴突，但没有外段的光感受器。在超微结构水平，锌定位于肌样区，线粒体内节，核间隙和细胞核的感光细胞。此外，在光感受器的末梢、视锥蒂、视杆小球以及OPL的某些突起中发现了银颗粒，可能是水平细胞的树突。锌在光感受器末梢的存在表明锌可能从光感受器末梢释放并在视网膜外层起调节作用。Synapse 62：352-357，2008.© 2008 Wiley利斯公司
Many lines of evidence suggest that zinc may play an important neuromodulatory role in the central nervous system, including the retina. In this work, localization of zinc in the outer retina of carp was studied, using the silver amplification method, by light and electron microscopy. Reaction products (silver grains) were widely distributed throughout the retina, including photoreceptors, the outer and inner nuclear layers (ONL and INL), the ganglion cell layer (GCL), as well as in both outer and inner plexiform layers (OPL and IPL). Generally, staining for zinc was stronger in the outer retina than in the inner retina, and grains were aggregated with the greatest density in the OPL and the outer limiting membrane (OLM). Silver precipitates were also detected in the inner segments, axons, but not outer segments of photoreceptors. At the ultrastructural level, zinc was localized to myoid regions, mitochondria in the inner segments, internuclear space and nuclei of photoreceptors. In addition, silver grains were found in the terminals of photoreceptors, cone pedicles, and rod spherules, as well as in some processes in the OPL, which might be dendrites of horizontal cells. The presence of zinc in the terminals of photoreceptors suggests that zinc might be released from photoreceptor terminals and play modulatory roles in the outer retina. Synapse 62:352–357, 2008. © 2008 Wiley‐Liss, Inc.