Xanthogenate nucleic acid isolation from cultured and environmental cyanobacteria
Xanthogenate nucleic acid isolation from cultured and environmental cyanobacteria
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DOI：
10.1046/j.1529-8817.2000.99079.x
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发表时间：
2000-02-01
影响因子：
2.9
通讯作者：
Neilan, BA
中科院分区：
文献类型：
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作者：
Tillett, D;Neilan, BA
事实证明，从蓝藻菌株（特别是环境分离株）中分离出高质量核酸绝非易事。我们提出了从一系列培养和环境蓝藻中提取高分子量 DNA 和 RNA 的新技术，这些蓝藻包括属于微囊藻属、Lyngbya、Pseudanabaena、Aphanizomenon、Nodularia、Anabaena 和 Nostoc 属的染色剂，基于使用无毒的多糖增溶化合物黄原酸酯。这些方法快速，不需要酶或机械细胞破碎，并且已用于分离不含酶抑制剂或核酸酶的 DNA 和 RNA。此外，这些程序已被证明对于水华形成和其他环境蓝藻分离株的分子分析至关重要。最后，这些技术对于各种非蓝细菌微生物（包括革兰氏阳性菌和革兰氏阴性菌以及古细菌）具有一般微生物学用途。
The isolation of high-quality nucleic acids from cyanobacterial strains, in particular environmental isolates, has proven far from trivial. We present novel techniques for the extraction of high molecular weight DNA and RNA from a range of cultured and environmental cyanobacteria, including stains belonging to the genera Microcystis, Lyngbya, Pseudanabaena, Aphanizomenon, Nodularia, Anabaena, and Nostoc, based on the use of the nontoxic polysaccharide solubilizing compound xanthogenate. These methods are rapid, require no enzymatic or mechanical cell disruption, and have been used to isolate both DNA and RNA free of enzyme inhibitors or nucleases. In addition, these procedures have proven critical in the molecular analysis of bloom-forming and other environmental cyanobacterial isolates. Finally, these techniques are of general microbiological utility for a diverse range of noncyanobacterial microorganisms, including Gram-positive and Gram-negative bacteria and the Archea.