Statistical tests for detecting gene conversion.
Statistical tests for detecting gene conversion.
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DOI：
10.1093/oxfordjournals.molbev.a040567
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发表时间：
1989-09
影响因子：
10.7
通讯作者：
S. Sawyer
中科院分区：
文献类型：
--
作者：
S. Sawyer
描述了用于检测同源DNA序列样本的基因转化的统计检验。这些测试是基于某一对序列一致的片段分布的不平衡。该方法自动控制沿基因组的可变突变率，并且不依赖于样本的潜在单系子集的先验选择。这些测试表明，在大肠杆菌的两个基因座上发生了多个基因内转换事件。在大肠杆菌中的GND基因座显示出长度为70-200个碱基的最大片段非常显著的过剩，这表明有这个大小的转换事件。数据还表明，这些短转换事件的发生率可能是中性突变率的量级。也有证据表明相邻密码子位置存在相关突变。同样的测试适用于RNA病毒中的一个基因座，结果为阴性。
Statistical tests for detecting gene conversion are described for a sample of homologous DNA sequences. The tests are based on imbalances in the distribution of segments on which some pair of sequences agrees. The methods automatically control for variable mutation rates along the genome and do not depend on a priori choices of potentially monophyletic subsets of the sample. The tests show strong evidence for multiple intragenic conversion events at two loci in Escherichia coli. The gnd locus in E. coli shows a highly significant excess of maximal segments of length 70-200 bp, which suggests conversion events of that size. The data also indicate that the rate of these short conversion events might be of the order of neutral mutation rate. There is also evidence for correlated mutation in adjacent codon positions. The same tests applied to a locus in an RNA virus were negative.