Determination of β-amyloid peptides in vitro aggregation process by electrochemistry
Determination of β-amyloid peptides in vitro aggregation process by electrochemistry
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电化学测定β-淀粉样肽体外聚集过程
DOI：
10.5246/jcps.2020.03.017
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发表时间：
2020
影响因子：
--
通讯作者：
Ling Xiaomei
中科院分区：
文献类型：
--
作者：
Zhang Sufang;Li Cong;Zhang Ying;Zou Juncheng;Cui Yinzhu;Ling Xiaomei
通过合成铈掺杂氧化锌（CeZnO）纳米粒子，修饰玻碳电极（GCE）固定抗体mAb，并用乙醇胺（EA）阻断未结合的羧基位点，成功构建了检测β-淀粉样肽（Aβ）的阻抗传感器（EA/mAb/CeZnO/GCE）。该传感器的线性检测范围为10 ~ 100 pM，检出限为1.7 pM(S/N = 3)，电极间和电极内的相对标准偏差（rsd）均小于3.0%。在4℃下保存2 d后，其阻抗值为原始阻抗响应的101.6%。该传感器用于测定体外培养的Aβ残留量。结果表明，孵育48 h和60 h后，聚类Aβ（低聚物和纤维）含量基本不变，达到90%左右；该方法具有省时、简便、经济、电导率好、重现性和稳定性好等优点。
An impedimetric sensor(EA/mAb/CeZnO/GCE)for detecting β-amyloid peptides(Aβ)was successfully constructed by synthesizing ceria doped zinc oxide(CeZnO)nanoparticles to modify the glassy carbon electrode(GCE)for immobilizing the antibody mAb,and blocking the unbound carboxyl sites with ethanolamine(EA).The sensor exhibited a linear detection range of 10-100 pM and a detection limit of 1.7 pM(S/N = 3),and the relative standard deviations(RSDs)of the inter-electrode and intra-electrodes were less than 3.0%.After it was stored at 4 °C for 2 d,its impedance value was 101.6% of original impedance response.The sensor was used to measure the remaining content of Aβ incubated in vitro.The results showed that after incubation for 48 h and 60 h,the content of aggregated Aβ(oligomers and fibers)almost unchanged,reaching about 90%.This method had the advantages of time-saving,simple procedure,economical,excellent conductivity,good reproducibility and stability.