Disruption of ppr3, ppr4, ppr6 or ppr10 induces flocculation and filamentous growth in Schizosaccharomyces pombe
Disruption of ppr3, ppr4, ppr6 or ppr10 induces flocculation and filamentous growth in Schizosaccharomyces pombe
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ppr3、ppr4、ppr6 或 ppr10 的破坏会诱导粟酒裂殖酵母絮凝和丝状生长
DOI：
10.1093/femsle/fny141
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发表时间：
2018-08-01
影响因子：
2.1
通讯作者：
Huang, Ying
中科院分区：
文献类型：
--
作者：
Su, Yang;Chen, Jie;Huang, Ying
五肽重复序列（PPR）蛋白是线粒体和叶绿体RNA代谢的主要参与者，这是细胞器正常功能所必需的。裂殖酵母有10个PPR蛋白。我们之前曾报道过，ppr 3、ppr 4、ppr 6或ppr 10的缺失会扰乱铁体内平衡，导致活性氧簇积累和细胞凋亡。在目前的研究中，我们发现ppr 3，ppr 4，ppr 6或ppr 10的缺失会导致细胞的无性絮凝和丝状生长。此外，在这些ppr缺失突变体中诱导了编码细胞表面絮凝蛋白和细胞壁重塑酶的许多基因的表达。我们还表明，三角洲ppr 10细胞，并在较小程度上，三角洲ppr 4和三角洲ppr 6细胞，表现出增加耐受H2 O2毒性相比，野生型菌株。最后，我们发现参与铁摄取和/或铁稳态的基因的过表达可能导致野生型细胞的絮凝。我们的研究结果表明，在突变体中由ppr 3，ppr 4，ppr 6或ppr 10的损失引起的细胞内铁水平的升高可能会导致絮凝和丝状生长在S。粟酒
Pentatricopeptide repeat (PPR) proteins are major players in mitochondrial and chloroplast RNA metabolism, which is essential for normal organellar function. The fission yeast Schizosaccharomyces pombe has 10 PPR proteins. We have previously reported that loss of ppr3, ppr4, ppr6 or ppr10 perturbs iron homeostasis leading to accumulation of reactive oxygen species and apoptotic cell death. In the present study, we show that loss of ppr3, ppr4, ppr6 or ppr10 can cause non-sexual flocculation and filamentous growth of cells. Furthermore, expression of a number of genes encoding cell-surface flocculins and cell wall-remodeling enzymes are induced in these ppr-deletion mutants. We also show that Delta ppr10 cells, and, to a lesser extent, Delta ppr4 and Delta ppr6 cells, exhibited increased tolerance to H2O2 toxicity compared with the wild-type strain. Finally, we found that overexpression of genes involved in iron uptake and/or iron homeostasis could cause the flocculation of wild-type cells. Our findings suggest that an elevated level of intracellular iron in the mutant caused by loss of ppr3, ppr4, ppr6 or ppr10 may result in flocculation and filamentous growth in S. pombe.