Buffered potassium permanganate-uranyl acetate-lead citrate staining seguence for ultrathin sections.
Buffered potassium permanganate-uranyl acetate-lead citrate staining seguence for ultrathin sections.
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超薄切片的缓冲高锰酸钾-醋酸双氧铀-柠檬酸铅染色序列。
DOI：
10.3109/10520297309116611
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发表时间：
1973
期刊：
影响因子：
--
通讯作者：
B. L. Soloff
中科院分区：
文献类型：
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作者：
B. L. Soloff
从大多数环氧树脂包埋物上切下的组织薄切片的有效电子染色是通过用磷酸盐缓冲的pH 6.5的0.9%KMnO4溶液处理安装在铜网格上的切片，然后用饱和在50%乙醇中的乙酸双氧铀溶液处理，然后用0.2%柠檬酸铅溶液处理。高锰酸盐染色后使用酸洗铜网格和磷酸盐缓冲液冲洗可最大限度地减少污染。有人建议，三重染色产生的电子密度的增加可能会导致部分从高锰酸钾诱导的蛋白质的改变，导致形成的可用的结合位点的醋酸铀酰和柠檬酸铅的数量增加。
An effective electron stain for thin sections of tissue cut from most epoxy embedments results from treatment of sections mounted on copper grids with a 0.9% KMnO4 solution buffered at pH 6.5 with phosphates, then by treatment with a solution of uranyl acetate saturated in 50% ethanol, followed by treatment in a 0.2% lead citrate solution. The use of acid-washed copper grids and a phosphate buffer rinse following permanganate staining minimizes contamination. It is suggested that the increase in electron density produced by the triple stain may result in part from KMnO4 induced alterations of protein that lead to the formation of an increased number of available bonding sites for the uranyl acetate and lead citrate.