Intracellular trehalose improves the survival of cryopreserved mammalian cells
Intracellular trehalose improves the survival of cryopreserved mammalian cells
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DOI：
10.1038/72608
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发表时间：
2000-02-01
影响因子：
46.9
通讯作者：
Toner, M
中科院分区：
文献类型：
--
作者：
Eroglu, A;Russo, MJ;Toner, M
我们报告，低浓度的细胞内海藻糖的引入可以大大提高哺乳动物细胞在冻存过程中的存活率。使用金黄色葡萄球菌α-溶血素的基因工程突变体在细胞膜中产生孔，我们能够将海藻糖加载到细胞中。低浓度（0.2 M）的海藻糖允许超过80%的3 T3成纤维细胞和70%的人角质形成细胞的长期解冻后存活。这些结果表明，简化和广泛适用的冷冻协议可能使用糖作为细胞内的冷冻保护添加剂。
We report that the introduction of low concentrations of intracellular trehalose can greatly improve the survival of mammalian cells during cryopreservation. Using a genetically engineered mutant of Staphylococcus aureus alpha-hemolysin to create pores in the cellular membrane, we were able to load trehalose into cells. Low concentrations (0.2 M) of trehalose permitted long-term post-thaw survival of more than 80% of 3T3 fibroblasts and 70% of human keratinocytes. These results indicate that simplified and widely applicable freezing protocols may be possible using sugars as intracellular cryoprotective additives.