FRET from single to multiplexed signaling events.
FRET from single to multiplexed signaling events.
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DOI：
10.1007/s12551-017-0252-z
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发表时间：
2017-04
影响因子：
--
通讯作者：
Wouters FS
中科院分区：
文献类型：
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作者：
Bunt G;Wouters FS
Förster共振能量转移（FRET）是一种强大的工具，用于可视化分子信号事件，如细胞中的蛋白质活性和相互作用。在其不同的实施方式中，FRET显微镜主要用于监测单个事件。最近，有一种趋势，扩展FRET成像对多个事件和相互作用的同时检测。实验复杂性的增加需要更深入地了解FRET的生物物理背景。一个供体的多个受体的存在影响了两个分子之间的FRET的众所周知的形式主义，通过被称为“天线”和“盈余”效应的机制增加了距离灵敏度。我们将讨论这些影响的性质，并提出已被用来解开多重FRET实验中的多蛋白质相互作用的组合转移率的成像方法。多路复用策略正在成为非常宝贵的分析工具，用于阐明生物复合物和可视化生理和病理条件下细胞信号网络中的决策点。
Förster resonance energy transfer (FRET) is a powerful tool for the visualization of molecular signaling events such as protein activities and interactions in cells. In its different implementations, FRET microscopy has been mainly used for monitoring single events. Recently, there has been a trend of extending FRET imaging towards the simultaneous detection of multiple events and interactions. The concomitant increase in experimental complexity requires a deeper understanding of the biophysical background of FRET. The presence of multiple acceptors for one donor affects the well-known formalism for FRET between two molecules, increasing distance sensitivity through mechanisms that have become known as the ‘antenna’ and ‘surplus’ effect. We will discuss the nature of these effects and present the imaging methods that have been used to unravel the combined transfer rates in the multi-protein interactions of multiplexed FRET experiments. Multiplexing strategies are becoming invaluable analytical tools for the elucidation of biological complexes and for the visualization of decision points in cellular signaling networks in physiological and pathological conditions.