Connexins regulate calcium signaling by controlling ATP release
Connexins regulate calcium signaling by controlling ATP release
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DOI：
10.1073/pnas.95.26.15735
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发表时间：
1998-12-22
影响因子：
11.1
通讯作者：
Nedergaard, M
中科院分区：
文献类型：
--
作者：
Cotrina, ML;Lin, JHC;Nedergaard, M
间隙连接蛋白，连接蛋白的强制表达，使间隙连接缺陷细胞系传播细胞间钙波。在这里，我们表明，ATP分泌的耦合不良的细胞系，C6胶质瘤，HeLa细胞，和U373胶质母细胞瘤，是增强连接蛋白表达的5至15倍。ATP释放需要嘌呤受体激活的细胞内Ca 2+动员，并被Cl-通道阻滞剂抑制。钙波的传播也被嘌呤能受体拮抗剂和Cl-通道阻滞剂降低，但对缝隙连接抑制剂不敏感。这些观察结果表明，与连接蛋白表达相关的细胞间信号传导是由ATP释放增强引起的，而不是像以前认为的那样，由细胞间偶联增加引起的。
Forced expression of gap junction proteins, connexins, enables gap junction-deficient cell lines to propagate intercellular calcium waves. Here, we show that ATP secretion from the poorly coupled cell lines, C6 glioma, HeLa, and U373 glioblastoma, is potentiated 5- to 15-fold by connexin expression. ATP release required purinergic receptor-activated intracellular Ca2+ mobilization and was inhibited by Cl- channel blockers. Calcium wave propagation also was reduced by purinergic receptor antagonists and by Cl- chan nel blockers but insensitive to gap junction inhibitors. These observations suggest that cell-to-cell signaling associated with connexin expression results from enhanced ATP release and not, as previously believed, from an increase in intercellular coupling.