Studies of the subgingival microflora in patients with acquired immunodeficiency syndrome.
Studies of the subgingival microflora in patients with acquired immunodeficiency syndrome.
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DOI：
10.1902/jop.1990.61.11.699
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发表时间：
1990-11
影响因子：
4.3
通讯作者：
J. Zambon;H. Reynolds;R. Genco
中科院分区：
文献类型：
--
作者：
J. Zambon;H. Reynolds;R. Genco
获得性免疫缺陷综合征(AIDS)患者中有两种独特的牙周病，HIV-牙周炎和HIV-牙周炎。为了解艾滋病患者牙周炎的病原菌种类，对11例艾滋病牙周炎患者的21份菌斑标本进行了优势可培养菌群检测。用免疫荧光法检测了50例艾滋病患者(其中32例为牙周炎患者)的128例龈下菌斑标本中伴生放线杆菌、中间类杆菌、牙龈卟啉单胞菌和直肠狼疮杆菌等牙周疑似病原体。在21个菌斑标本中分离出的666株细菌中，血链球菌II是最常见的菌株，占总数的18.5%，其次是嗜酸乳杆菌(12.2%)、牙龈卟啉单胞菌(12%)、核梭杆菌(11.4%)、表皮葡萄球菌(8.7%)、奈氏放线菌(7.5%)和粘性放线菌(4.7%)。核梭杆菌是最常见的种类，在76%的部位和91%的患者中发现。还发现了禽肠球菌和粪肠球菌、梭状芽孢杆菌和艰难梭菌以及肺炎克雷伯菌等肠道细菌。免疫荧光检测发现，在牙周炎患者和牙周炎患者中，伴生放线菌、中间芽孢杆菌和牙龈假单胞菌的携带率相似，而牙周炎患者的直肠杆菌携带率是直肠炎患者的四倍。在这些艾滋病患者中，龈下酵母菌是一种常见的发现，存在于62%的受试者和55%的部位。这项研究表明，艾滋病合并牙周炎患者的牙周菌斑中可能含有与非HIV感染者牙周炎相关的高比例牙周病原体，以及高比例的条件致病菌。
Two unique forms of periodontal disease, HIV-gingivitis and HIV-periodontitis, have been described in patients with Acquired Immunodeficiency Syndrome (AIDS). In order to determine the bacterial species associated with periodontitis in AIDS patients, the predominant cultivable microflora was examined in 21 subgingival plaque samples from 11 AIDS patients with periodontitis. The presence of putative periodontal pathogens including Actinobacillus actinomycetemcomitans, Bacteroides intermedius, Porphyromonas gingivalis (formerly B. gingivalis), and Wolinella recta was examined by immunofluorescence in 128 subgingival dental plaque samples from 50 AIDS patients including 32 patients with periodontitis. Of 666 bacterial strains isolated from the 21 subgingival plaque samples, Streptococcus sanguis II was the most frequently recovered species comprising 18.5% of the total number of isolates followed by Lactobacillus acidophilus (12.2%), Porphyromonas gingivalis (12%), Fusobacterium nucleatum (11.4%), Staphylococcus epidermidis (8.7%), Actinomyces naeslundii (7.5%), and Actinomyces viscosus (4.7%). Fusobacterium nucleatum was the most prevalent species and was found in 76% of the sites and 91% of the patients. Enteric species including Enterococcus avium and Enterococcus faecalis, Clostridium clostridiiforme and Clostridium difficle as well as Klebsiella pneumoniae also were recovered. Immunofluorescence assays detected similar carriage rates of A. actinomycetemcomitans, B. intermedius, and P. gingivalis in both gingivitis patients and periodontitis patients, while four times more periodontitis patients demonstrated W. recta. Subgingival yeast was a frequent finding in these AIDS patients, present in 62% of the subjects and 55% of the sites. This study indicates that subgingival plaque in AIDS patients with periodontitis can harbor high proportions of the same periodontal pathogens as are associated with periodontitis in non-HIV infected subjects as well as high proportions of opportunistic pathogens.