LuxS affects biofilm maturation and detachment of the periodontopathogenic bacterium Eikenella corrodens
LuxS affects biofilm maturation and detachment of the periodontopathogenic bacterium Eikenella corrodens
复制标题
DOI：
10.1016/j.jbiosc.2013.03.013
复制
发表时间：
2013-09-01
影响因子：
2.8
通讯作者：
Azakami, Hiroyuki
中科院分区：
文献类型：
--
作者：
Karim, Mohammad Minnatul;Hisamoto, Tatsunori;Azakami, Hiroyuki
先前，我们报道了腐蚀艾肯氏菌的生物膜形成受自诱导物-2（AI-2）的调节，基于Delta luxS突变体的生物膜形成效率大于野生型的观察（Azakami et al.，J. Biosci.生物工程，102，110-117，2006）。为了确定AI-2分子是否直接影响生物膜形成，我们将纯化的AI-2加入到luxS突变体和野生型E.腐蚀并通过使用静态测定比较生物膜形成。结果表明，E.通过添加Al-2增强了腐蚀性。我们还通过扫描电镜和激光共聚焦扫描显微镜比较了luxS突变体和野生型在流动细胞系统中形成的生物膜。与野生型相比，luxS突变体生物膜中的活菌数量显著减少并且分布更稀疏，这表明AI-2可能增强成熟生物膜。相反，通过改进的共聚焦反射显微镜的进一步分析表明，野生型生物膜比luxS突变体更早成熟，并且随着时间的推移变得更薄，分布更稀疏。这些数据表明，LuxS可能促进E.腐蚀(C)2013年，生物技术学会，日本。All rights reserved.
Previously, we reported that biofilm formation of Eikenella corrodens is regulated by autoinducer-2 (AI-2), based on observations that biofilm-forming efficiency of Delta luxS mutant was greater than that of the wild type (Azakami et al., J. Biosci. Bioeng., 102, 110-117, 2006). To determine whether the AI-2 molecule affects biofilm formation directly, we added purified AI-2 to luxS mutant and wild-type E. corrodens and compared biofilm formations by using a static assay. Results indicated that biofilm formation in E. corrodens was enhanced by the addition of AI-2. We also compared the biofilms formed by flow cell system for the luxS mutant and the wild type by using scanning electron microscopy and confocal laser scanning microscopy. The number of viable bacteria in the luxS mutant biofilm was dramatically reduced and more sparsely distributed than that of the wild type, which suggested that AI-2 might enhance the mature biofilm. Conversely, further analysis by modified confocal reflection microscopy indicated that the wild-type biofilm was matured earlier than that of the luxS mutant, and became thinner and more sparsely distributed with time. These data suggest that LuxS may facilitate the maturation and detachment of biofilm in E. corrodens. (C) 2013, The Society for Biotechnology, Japan. All rights reserved.