Ferrets develop fatal influenza after inhaling small particle aerosols of highly pathogenic avian influenza virus A/Vietnam/1203/2004 (H5N1)
Ferrets develop fatal influenza after inhaling small particle aerosols of highly pathogenic avian influenza virus A/Vietnam/1203/2004 (H5N1)
复制标题
DOI：
10.1186/1743-422x-7-231
复制
发表时间：
2010-09-15
期刊：
影响因子：
4.8
通讯作者：
Swayne, David E.
中科院分区：
文献类型：
--
作者：
Lednicky, John A.;Hamilton, Sara B.;Swayne, David E.
背景：关于H5N1流感病毒在人与人之间传播的潜在途径，目前了解有限，也不清楚人类是否会通过吸入雾化H5N1病毒颗粒而感染。在流感致病性和传播性研究中，雪貂经常被用作人类的动物模型。在这篇论文中，最近开发和验证的鼻式生物气溶胶吸入暴露系统被用于雪貂雾化A/越南/1203/2004(H5N1)病毒的吸入暴露研究。结果：通过鼻内滴注或吸入4种不同剂量的流感病毒A/越南/1203/2004(H5N1)，均可成功感染雪貂。每只雪貂鼻内或吸入101、102、103或104个传染性病毒颗粒后，动物出现严重的流行性脑脊髓炎。结论：甲型H5N1流感病毒对雪貂具有高度的传染性和致死性。吸入雾化病毒感染的动物比通过鼻内滴注感染的动物出现临床症状的时间更早。
Background: There is limited knowledge about the potential routes for H5N1 influenza virus transmission to and between humans, and it is not clear whether humans can be infected through inhalation of aerosolized H5N1 virus particles. Ferrets are often used as a animal model for humans in influenza pathogenicity and transmissibility studies. In this manuscript, a nose only bioaerosol inhalation exposure system that was recently developed and validated was used in an inhalation exposure study of aerosolized A/Vietnam/1203/2004 (H5N1) virus in ferrets. The clinical spectrum of influenza resulting from exposure to A/Vietnam/1203/2004 (H5N1) through intranasal verses inhalation routes was analyzed.Results: Ferrets were successfully infected through intranasal instillation or through inhalation of small particle aerosols with four different doses of Influenza virus A/Vietnam/1203/2004 (H5N1). The animals developed severe influenza encephalomyelitis following intranasal or inhalation exposure to 101, 102, 103, or 104 infectious virus particles per ferret.Conclusions: Aerosolized Influenza virus A/Vietnam/1203/2004 (H5N1) is highly infectious and lethal in ferrets. Clinical signs appeared earlier in animals infected through inhalation of aerosolized virus compared to those infected through intranasal instillation.