Estradiol reference intervals in women during the menstrual cycle, postmenopausal women and men using an LC-MS/MS method
Estradiol reference intervals in women during the menstrual cycle, postmenopausal women and men using an LC-MS/MS method
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DOI：
10.1016/j.cca.2019.04.062
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发表时间：
2019-08-01
影响因子：
5
通讯作者：
Heijboer, Annemieke C.
中科院分区：
文献类型：
--
作者：
Verdonk, Sara J. E.;Vesper, Hubert W.;Heijboer, Annemieke C.
背景：为了最佳的医疗决策，需要不同年龄和性别的雌二醇的统一参考区间。我们的目的是建立一个高度准确的和可追溯的LC-MS/MS方法的参考区间，并比较这些参考区间在literature.Methods：雌二醇测定在月经周期中每天获得的血清中的30名健康的绝经前妇女和64名男性和33名绝经后妇女的血清。通过与CDC参考方法的方法比较，证明了我们的LC-MS/MS方法的准确性。早期卵泡期的排卵参考间期（第-15至-6天）为31-771 pmol/L;在卵泡晚期（第-5至-1天）104-1742 pmol/L; LH峰值期间（第0天）275-2864 pmol/L;黄体早期期间（第+1至+4天）95-1188 pmol/L;黄体中期（第+5至+9天）151-1941 pmol/L;黄体晚期（第+10至+14天）39-1769 pmol/L。男性的参考区间为12-136 pmol/L，绝经后女性< 26 pmol/L。结论：建立的雌二醇参考区间可用于所有可追溯的LC-MS/MS方法，用于医疗决策。
Background: For optimal medical decision-making, harmonized reference intervals for estradiol for different ages and both sexes are needed. Our aim was to establish reference intervals using a highly accurate and traceable LC-MS/MS method and to compare these with reference intervals in literature.Methods: Estradiol was measured in serum obtained daily during the menstrual cycle of 30 healthy premenopausal women and in serum of 64 men and 33 postmenopausal women. The accuracy of our LC-MS/MS method was demonstrated by a method comparison with the CDC reference method.Results: Our LC-MS/MS method was traceable to the reference method. Estradiol reference interval during the early follicular phase (days -15 to -6) was 31-771 pmol/L; during the late follicular phase (days -5 to -1) 104-1742 pmol/L; during the LH peak (day 0) 275-2864 pmol/L; during the early luteal phase (days +1 to +4) 95-1188 pmol/L; during mid luteal phase (days +5 to +9) 151-1941 pmol/L; during late luteal phase (days +10 to + 14) 39-1769 pmol/L. The reference interval for men was 12-136 pmol/L and for postmenopausal women < 26 pmol/L.Conclusions: The established estradiol reference intervals can be used for all traceable LC-MS/MS methods for medical-decision making.