Simultaneous carbon-nitrogen removal in wastewater using phosphorylated PVA-immobilized microorganisms

Simultaneous carbon-nitrogen removal in wastewater using phosphorylated PVA-immobilized microorganisms
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DOI:
10.1016/s0141-0229(98)00054-4
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发表时间:
1998-10-01
影响因子:
3.4
通讯作者:
Houng, JY
Houng, JY
中科院分区:
工程技术3区
文献类型:
--
作者:
Chen, KC;Lee, SC;Houng, JY

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驯化污泥固定化的磷酸化聚乙烯醇(PVA)凝胶。固定化细胞反应器系统建立在一个12-2的连续曝气容器与合成的城市污水作为种子基板。此外,建立了一种通过检测微生物细胞蛋白质含量来估算悬浮污泥和固定化细胞珠的生物量浓度的方法。系统运行水力停留时间为2-10 h,COD负荷为0.855-4.223 g COD l(-1)d(-1)。当COD负荷低于2.0gCOD l(-1)d(-1)时,COD去除率可达90%以上,总氮去除率在45%左右。固定化细胞方法已经产生了非常有前途的结果,特别是在保持高生物质浓度以获得高效率和减少剩余污泥的产生以降低操作成本方面。通过测量凝胶弯曲部的硝酸盐排出活性,实验验证了凝胶珠内部发生的所谓的曝气反硝化。显微镜观察显示,三种细菌物种,即,BOD氧化剂、硝化剂和硝化剂从凝胶珠的外围表面到内部形成了栖息地隔离。(C)1998年爱思唯尔科学公司
Acclimated sludge was immobilized by a previously developed phosphorylated polyvinyl alcohol (PVA) gel. The immobilized-cell reactor system was established in a 12-2 continuous aeration vessel with a synthetic municipal wastewater as the Seed substrate. In addition, a method was developed to estimate the biomass concentration of the suspended sludge and the immobilized-cell beads by detecting the microbial cell protein content. The system was operated at a HRT of 2-10 h in which the COD loading rate ranged from 0.855-4.223 g COD l(-1) d(-1). More than 90% of COD removed efficiency was obtained at a COD loading rate lower than 2.0 g COD l(-1) d(-1) accompanied by a total nitrogen removal efficiency at around 45%. The immobilized cell process has yielded highly promising results particularly in terms of maintaining a high biomass concentration to attain high efficiency and reducing production of excess sludge to decrease operation cost. The so-called aerated denitrification occurring inside the gel beads was verified experimentally by measuring the nitrate I eduction activity of gel bends. Microscopic observation revealed that three kinds of bacterial species, i.e., BOD oxidizers, nitrifiers, and denitrifiers, developed a habitat segregation from the peripheral surface into the interior part of the gel bead. (C) 1998 Elsevier Science Inc.