Reclassification of phenotypically identified Staphylococcus intermedius strains
Reclassification of phenotypically identified Staphylococcus intermedius strains
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DOI：
10.1128/jcm.00360-07
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发表时间：
2007-09-01
影响因子：
9.4
通讯作者：
Hiramatsu, Keiichi
中科院分区：
文献类型：
--
作者：
Sasaki, Takashi;Kikuchi, Ken;Hiramatsu, Keiichi
为了对表型鉴定的中间葡萄球菌（可能包括真中间葡萄球菌和假中间葡萄球菌和德尔菲尼葡萄球菌等新种）进行重新分类，我们分析了117株中间葡萄球菌群（SIG）的分子系统发育和表型特征，这些菌株通过快速ID32葡萄球菌试验初步鉴定为中间葡萄球菌。通过sodA和hsp60序列的系统发育分析，将SIG菌株划分为3个集群，分别属于S. pseudintermedius LMG 22219(T)、S. intermedius ATCC 29663 T和S. delphini LMG 22190(T)。所有来自狗、猫和人的SIG菌株均被鉴定为假中间球菌。除1株野鸽外，其余均为中间链球菌，所有家鸽、1株野鸽、1匹马和1只水貂的毒株均为德尔菲链球菌。此外，nuc基因的系统发育分析表明，S， delphini菌株分为两个集群，一个集群（S. delphini group）属于S. delphini LMG 22190T，另一个集群（S. delphini group）属于S. delphini LMG 22190T，而不是S. delphini LMG 22190T。DNA-DNA杂交结果表明，B组菌株与A组、中间链球菌和伪中间链球菌有明显区别。通过精氨酸二水解酶阳性和β -菌糖和d -甘露醇的产酸能力，可以将中间葡萄球菌与假中间葡萄球菌或德尔菲葡萄球菌区分。然而，没有发现飞燕草A组、飞燕草B组和假中间飞燕草的表型特征。结果表明，SIG菌株被重新划分为4个聚类，其中3个已建立种，1个可能是新种。
To reclassify phenotypically identified Staphylococcus intermedius strains, which might include true S. intermedius strains and novel species such as Staphylococcus pseudintermedius and Staphylococcus delphini, we analyzed molecular phylogenies and phenotypic characteristics of 117 S. intermedius group (SIG) strains tentatively identified as being S. intermedius by the Rapid ID32 Staph assay. From phylogenetic analyses of sodA and hsp60 sequences, the SIG strains were divided into three clusters, which belonged to S. pseudintermedius LMG 22219(T), S. intermedius ATCC 29663 T, and S. delphini LMG 22190(T). All the SIG strains from dogs, cats, and humans were identified as being S. pseudintermedius. The wild pigeon strains, except one, were identified as being S. intermedius, and strains from all domestic pigeons, one wild pigeon, horses, and a mink were identified as being S. delphini. In addition, a phylogenetic analysis of nuc genes revealed that S, delphini strains were divided into two clusters: one was the cluster (S. delphini group A) that belonged to S. delphini LMG 22190T, and the other was the cluster (S. delphini group B) that was more related to S. pseudintermedius LMG 22219T than S. delphini LMG 22190T. The DNA-DNA hybridization results showed that S. delphini group B strains were distinguished from S. delphini group A, S. intermedius, and S. pseudintermedius strains. S. intermedius is distinguishable from S. pseudintermedius or S. delphini by positive arginine dihydrolase and acid production from beta-gentiobiose and D-mannitol. However, phenotypical characteristics to differentiate S. delphini group A, S. delphini group B, and S. pseudintermedius were not found. In conclusion, SIG strains were reclassified into four clusters with three established and one probably novel species.