Two forms of potentially lethal damage have similar repair kinetics in plateau- and in log-phase cells.
Two forms of potentially lethal damage have similar repair kinetics in plateau- and in log-phase cells.
复制标题
两种形式的潜在致命损伤在平台期和对数期细胞中具有相似的修复动力学。
DOI：
10.1080/09553008514550801
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Elkind,MM
中科院分区：
文献类型：
--
作者：
Utsumi,H;Elkind,MM
在生长的平台期和对数期检查两种不同后处理对 X 照射的中国仓鼠细胞（V79 系）存活的影响。两个生长期的细胞都获得了类似的定性结果；也就是说，用高渗磷酸盐缓冲盐水进行后处理可实现类似的存活率降低，并且用条件培养基进行后处理可实现类似的存活率增加。此外，在两种细胞中，修复过程的动力学相似，尽管这两种过程的动力学彼此相当不同。虽然结果表明潜在致命损伤的修复类型和/或途径可能存在本质差异，但它们也说明了该术语比通常情况更广泛的含义。考虑到可能致命的 DNA 损伤量，得出的结论是，作为本研究主题的两种类型的损伤仅占潜在致命损伤总量的一小部分。
The effect on the survival of X-irradiated Chinese hamster cells (line V79) of two different post-treatments is examined in plateau- and in log-phases of growth. Qualitatively similar results are obtained with cells in both growth phases; that is, similar reductions in survival are effected by post-treatments with hypertonic phosphate buffered saline, and similar increases in survival are effected by post-treatments with conditioned medium. In addition, in both kinds of cells the kinetics of the repair processes are similar even though the kinetics of the two processes differ from each other considerably. While the results indicate that there can be essential differences in the type and/or the pathways of repair of potentially lethal damage, they also illustrate a broader meaning of this term than has been customary. Considered relative to the amount of DNA damage that can be expected to be potentially lethal, it is concluded that the two types of damage that are the subjects of this study represent only small sectors of the total amount of potentially lethal damage.