Sol-gel materials as efficient enzyme protectors: Preserving the activity of phosphatases under extreme pH conditions
Sol-gel materials as efficient enzyme protectors: Preserving the activity of phosphatases under extreme pH conditions
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DOI：
10.1021/ja0507719
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发表时间：
2005-06-08
影响因子：
15
通讯作者：
Avnir, D
中科院分区：
文献类型：
--
作者：
Frenkel-Mullerad, H;Avnir, D
通过包埋在（表面活性剂改性的）硅溶胶-凝胶基质中，碱性磷酸酶（AIP）在pH 9.5时自然具有最佳活性-在低至pH 0.9的极端酸性环境中保持功能，酸性磷酸酶（AcP）-在pH 4.5时自然具有最佳活性-在极端碱性环境中保持功能，高达pH 13.0。提供的命题的起源的基质提供这种高效的保护和作为的起源的协同增强效应时，二氧化硅和表面活性剂被用作一个组合的截留环境的能力。发现酶对苛刻pH值的保护能力取决于表面活性剂的性质。
By entrapment in (surfactant modified) silica sol-gel matrixes, alkaline phosphatase (AIP) naturally with optimum activity at pH 9.5 - was kept functioning at extreme acidic environments as low as pH 0.9, and acid phosphatase (AcP) - naturally with optimum activity at pH 4.5 - was kept functioning at extreme alkaline environments, up to pH 13.0. Propositions are offered as to the origin of the ability of the matrixes to provide such highly efficient protection and as to the origin of the synergetic enhancing effect when both the silica and the surfactants are used as a combined entrapping environment. It was found that the protectability of the enzymes against harsh pH values is dependent on the nature of the surfactant.