Chronic viral hepatitis induced by hepatitis C but not hepatitis B virus infection correlates with increased liver angiogenesis
Chronic viral hepatitis induced by hepatitis C but not hepatitis B virus infection correlates with increased liver angiogenesis
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DOI：
10.1053/jhep.1997.v25.pm0008985296
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发表时间：
1997-01-01
期刊：
影响因子：
13.5
通讯作者：
Ziche, M
中科院分区：
文献类型：
--
作者：
Mazzanti, R;Messerini, L;Ziche, M
慢性B肝炎病毒（HBV）和丙型肝炎病毒（HCV）感染导致肝硬化并增加肝细胞癌（HCC）发展的风险。血管生成是肿瘤发生的一个重要步骤，并有助于成人器官中的肿瘤进展;然而，慢性病毒性肝炎期间肝脏中血管生成发生的程度尚未研究。99例匹配的HBV或HCV慢性肝炎患者与13例对照组（5例患者为家族性高胆红素血症，肝组织学正常; 6例患者为II期原发性胆汁性肝硬化; 2例患者为假性炎性肿瘤）一起研究。通过使用两种不同的抗内皮细胞抗原抗体（QB-END/10和因子VIII）进行免疫染色，在肝活检中评估微血管密度。此外，检测了HCV或HBV阳性患者和对照的肝匀浆和血清在体外刺激新鲜分离的人内皮细胞迁移和增殖的能力。与HBV感染者或对照组相比，HCV阳性患者血管生成的证据明显更频繁（74% vs. 39% vs. 8%）（卡方2 = 20.78; P
Chronic hepatitis B virus (HBV) and hepatitis C virus (HCV) infections lead to cirrhosis and increase the risk for the development of hepatocellular carcinoma (HCC). Angiogenesis is an essential step in oncogenesis and contributes to tumor progression in adult organs; however, to what extent angiogenesis occurs in the liver during chronic viral hepatitis has not been studied. Ninety-nine matched patients affected by chronic hepatitis due to either HBV or HCV were studied together with 13 controls (5 patients were affected by familial hyperbilirubinemia with normal liver histology; 6 patients with stage II primary biliary cirrhosis; and 2 patients with pseudo inflammatory tumor). Microvessel density was assessed in liver biopsies by immunostaining using two different antibodies against endothelial cell antigens, QB-END/10 and Factor VIII. In addition, the liver homogenates and sera of HCV- or HBV-positive patients and controls were tested for their capacity to stimulate the migration and proliferation of freshly isolated human endothelial cells in vitro. Evidence of angiogenesis was significantly more frequent in HCV-positive patients compared with HBV-infected subjects or controls (74% vs. 39% vs. 8%) (chi(2) = 20.78; P