Cross-bridge apparent rate constants of human gallbladder smooth muscle.
Cross-bridge apparent rate constants of human gallbladder smooth muscle.
复制标题
人胆囊平滑肌的跨桥表观速率常数。
DOI：
10.1007/s10974-011-9260-y
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发表时间：
2011
影响因子：
2.7
通讯作者：
Li WG
中科院分区：
文献类型：
--
作者：
Li WG
本文研究了人胆囊平滑肌的收缩。一个两状态的跨桥模型被用来估计的表观附着和脱离速率常数，以及增加的钙离子浓度从峰值主动应力在等长收缩。根据完全基于非侵入性常规超声扫描的机械模型计算主动应力。在两个状态的跨桥模型中，两个表观速率常数，代表总的附着/分离的跨桥，分别估计使用主动应力预测51名受试者接受胆囊收缩素激发试验，连同估计从四个状态的跨桥模型的猪颈动脉，牛气管和豚鼠GB平滑肌。该研究表明，表观速率常数应具有患者特异性，即应激水平较低的患者的表观速率常数较小。换句话说，患病的GB可能需要发展快速循环的跨桥来补偿排空过程。这是对GB疼痛进行更定量和非侵入性测量的第一步，并可能为了解GB运动和开发有效的药物治疗提供有用的见解。
This paper studies human gallbladder (GB) smooth muscle contractions. A two-state cross-bridge model was used to estimate the apparent attachment and detachment rate constants, as well as increased Ca2+concentration from the peak active stress during the isometric contraction. The active stress was computed from a mechanical model based entirely on non-invasive routine ultrasound scans. In the two-state cross-bridge model, the two apparent rate constants, representing the total attached/detached cross-bridges, respectively, were estimated using active stress prediction for 51 subjects undergoing cholecystokinin-provocation test, together with estimates from the four-state cross-bridge model for a swine carotid, bovine tracheal and guinea pig GB smooth muscles. The study suggests that the apparent rate constants should be patient-specific, i.e. patients with a lower stress level are characterized by smaller apparent rate constants. In other words, the diseased GB may need to develop fast cycling cross-bridges to compensate in the emptying process. This is a first step towards more quantitative and non-invasive measures of GB pain, and may provide useful insight in understanding GB motility and developing effective drug treatments.