Comparison of post-translational modifications of alpha A-crystallin from normal and hereditary cataract rats
Comparison of post-translational modifications of alpha A-crystallin from normal and hereditary cataract rats
复制标题
DOI：
10.1007/s00726-003-0050-8
复制
发表时间：
2004-03-01
期刊：
影响因子：
3.5
通讯作者：
Saito, T
中科院分区：
文献类型：
--
作者：
Fujii, N;Takeuchi, N;Saito, T
为了探讨透镜混浊与透镜蛋白质各种修饰的关系，我们分析和比较了85日龄正常Wistar大鼠和遗传性白内障模型ICR/f大鼠的透镜蛋白质的性质。本研究确定了正常和突变透镜蛋白之间的许多差异。在ICR/f突变大鼠中，γ-晶状体蛋白的相对量减少，高分子量（HMW）蛋白增加。在突变型ICR/f大鼠中观察到α A-晶状体蛋白的Asp-151的消旋和异构化，并且α A-晶状体蛋白的Met-1被氧化为甲硫氨酸亚砜。在年龄匹配的正常大鼠中未发现这些修饰。这些趋势与老化和白内障的人类晶状体一致。
In order to investigate the relationship between lens opacities and the various modifications of lens proteins, we analyzed and compared the properties of lens proteins of 85-day old normal Wistar rats and the hereditary cataract model, ICR/f rats. The present study identified many differences between normal and mutant lens proteins. In the ICR/f mutant rats, the relative amounts of gamma-crystallin decreased and high molecular weight (HMW) protein increased. Racemization and isomerization of Asp-151 of alpha A-crystallin was observed in the mutant ICR/f rats, and Met-1 of alpha A-crystallin was oxidized to methionine sulfoxide. These modifications were not found in the age-matched normal rats. These tendencies are consistent with aged and cataractous human lenses.