Estrogen effects of Daldinia concentrica and Psathyrella efflorescens extracts in vitro
Estrogen effects of Daldinia concentrica and Psathyrella efflorescens extracts in vitro
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DOI：
10.1016/j.jep.2007.11.018
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发表时间：
2008-02-28
影响因子：
5.4
通讯作者：
Thieulant, Marie-Lise
中科院分区：
文献类型：
--
作者：
Benie, Tanon;Kouakou, Koffi;Thieulant, Marie-Lise
达丁氏菌和风化假单胞菌是非洲传统医药中使用的两种真菌。在本研究中，它们的提取物在雌激素或雄激素依赖的细胞系中以类固醇依赖的转录活性和细胞增殖为终点来评估它们的类固醇活性。人乳腺癌MCF-7细胞在没有17β-雌二醇(E2)的情况下，用15或30微克/毫升的黄花丹参或风化假单胞菌提取物处理后，显著增加了雌激素受体(ER)依赖的报告基因的转录活性，与E2的范围相同。促性腺激素细胞系α-T3-1也获得了类似的数据。所有的作用都被纯雌激素拮抗剂ICI182,780所阻止。在没有添加类固醇的情况下，这两种提取物通过大约100%的E2反应诱导ER依赖的MCF-7和Ishikawa Var-I细胞系的细胞增殖。在后两种细胞系中，E2联合处理均未显示出竞争或相加效应。有趣的是，这些提取物在雄激素受体(AR)阳性和ER阴性的MDA-MB231细胞中没有雄激素样反应，这表明真菌的作用是雌激素特有的，提取物在所用浓度下是无毒的。这些结果提供了证据，证明达丁草或风化假单胞菌提取物在ER阳性细胞系中诱导雌激素样效应，这可能是体内观察到的效应的原因。(C)2007爱思唯尔爱尔兰有限公司。保留所有权利。
Daldinia concentrica and Psathyrella efflorescens are two fungi used in African traditional medicine. In the present study, their extracts were evaluated for their steroid activities in estrogen- or androgen-dependent cell lines using as endpoints steroid-dependent transcriptional activity and cell proliferation. Treatment of human breast cancer MCF-7 cells with 15 or 30 mu g/ml of Daldinia concentrica or Psathyrella efflorescens extracts in the absence of 17 beta-estradiol (E2) significantly increased the transcriptional activity of an estrogen receptor (ER)-dependent reporter gene, in the same range as E2. Similar data were obtained in gonadotrope cell line alpha-T3-1. All the effects were prevented by the pure estrogen antagonist, ICI 182,780. In the absence of steroid addition, the two extracts induced cell proliferation of ER-dependent MCF-7 and Ishikawa Var-I cell lines by approximately 100% of the E2 response. Combination treatments with E2 showed no competitive or additive effects in the two latter cell lines. Interestingly, the extracts had no androgen-like response in androgen receptor (AR)-positive and ER-negative MDA-MB231 cells, suggesting that fungi effects are estrogen specific and extracts are not toxic at used concentrations. Results provided evidence that Daldinia concentrica or Psathyrella efflorescens extracts induce estrogen-like effects in ER-positive cell lines, which could be responsible of the effects observed in vivo. (C) 2007 Elsevier Ireland Ltd. All rights reserved.