Three-dimensional culture and identification of human eccrine sweat glands in matrigel basement membrane matrix
Three-dimensional culture and identification of human eccrine sweat glands in matrigel basement membrane matrix
复制标题
基质胶基底膜基质中人体小汗腺的三维培养与鉴定
DOI：
10.1007/s00441-013-1718-3
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发表时间：
2013-12-01
影响因子：
3.6
通讯作者：
Fu, Xiaobing
中科院分区：
文献类型：
--
作者：
Li, Haihong;Chen, Lu;Fu, Xiaobing
细胞外基质 (ECM) 和上皮细胞之间的相互作用对于上皮的正常组织和功能是必要的。在本研究中，我们表明，在基质胶（ECM成分的代表）中培养的人小汗腺上皮细胞构成了研究人小汗腺三维重建、伤口修复以及再生和分化的良好模型。在基质胶中，来自人小汗腺的上皮细胞形成管状结构，然后管状结构卷曲成球状形状，其结构类似于体内的人小汗腺。一个球形形状可以连接到另一个球形形状或通过管状结构连接到细胞单层。苏木精和伊红染色显示管状结构具有位于外周的单层或复层上皮细胞和位于中心的管腔，分别类似于皮肤组织切片中的小汗腺的分泌部分或导管部分。培养物的免疫组织化学分析表明，细胞表达 CK7、CK19、上皮膜抗原和肌动蛋白。因此，基质胶促进上皮细胞从人小汗腺组织和分化为小汗腺组织。
Interactions between the extracellular matrix (ECM) and epithelial cells are necessary for the proper organization and function of the epithelium. In the present study, we show that human eccrine sweat gland epithelial cells cultured in matrigel, a representation of ECM components, constitute a good model for studying three-dimensional reconstruction, wound repair and regeneration and differentiation of the human eccrine sweat gland. In matrigel, epithelial cells from the human eccrine sweat gland form tubular-like structures and then the tubular-like structures coil into sphere-like shapes that structurally resemble human eccrine sweat glands in vivo. One sphere-like shape can be linked to another sphere-like shape or to a cell monolayer via tubular-like structures. Hematoxylin and eosin staining has revealed that the tubular-like structures have a single layer or stratified epithelial cells located peripherally and a lumen at the center, similar to the secretory part or duct part, respectively, of the eccrine sweat gland in sections of skin tissue. Immunohistochemical analysis of the cultures has demonstrated that the cells express CK7, CK19, epithelial membrane antigen and actin. Thus, matrigel promotes the organization and differentiation of epithelial cells from the human eccrine sweat gland into eccrine sweat gland tissues.