Quantification of human adenovirus and norovirus in river water in the north-east of France
Quantification of human adenovirus and norovirus in river water in the north-east of France
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DOI：
10.1007/s11356-018-3045-4
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发表时间：
2018-10-01
影响因子：
5.8
通讯作者：
Bertrand, Isabelle
中科院分区：
文献类型：
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作者：
Sedji, Maryse Iris;Varbanov, Mihayl;Bertrand, Isabelle
人类腺病毒（HAdVs）是一种主要的感染原因，已被提出作为水质的病毒指标。人诺如病毒（NoV）是病毒性急性胃肠炎的主要病因。需要关于这两种病毒在环境中流行的定量数据。在法国东北部的一条河流中，对hadv肠道腺病毒41型（HAdV41）和诺如病毒基因组I和基因组II （NoV GGI和GGII）的基因组进行了为期6个月的量化。样本是从污水处理厂排放的下游收集的。采用玻璃棉法浓缩病毒，采用数字液滴PCR （ddPCR）对病毒基因组进行定量分析。所有江河水样（15/15）HAdVs、HAdV41、NoV GGI和NoV GGII基因组均呈阳性。HAdVs、HAdV41和NoV GII基因组的浓度相似，HAdV41占HAdVs的80%。采用细胞培养-定量PCR方法（ICC-qPCR）对这些样品中的感染性hadv进行定量分析；检出率为93%(14/15)，定量率为53%（8/15）。因此，感染性hav占ddPCR检测到的hav颗粒总数的0.3 ~ 12.2%。73%（11/15）的样本中检出具有传染性的HAdV41颗粒。致病性肠道病毒的普遍存在凸显了废水排放对地表水质量的影响，并可能对人类健康构成威胁。HAdV41基因组的相对丰度强调了对其沿水循环的感染形式进行特异性检测的研究的必要性。
Human adenoviruses (HAdVs) are a major cause of infection and have been proposed as viral indicators of water quality. Human noroviruses (NoV) are the main cause of viral acute gastroenteritis. Quantitative data on the environmental prevalence of both viruses are needed. The genomes of HAdVs enteric adenovirus type 41 (HAdV41) and noroviruses of genogroups I and II (NoV GGI and GGII) were quantified over a 6-month period in a river located in north-eastern France. The samples were collected downstream from the discharge of a wastewater treatment plant. The viruses were concentrated using a glass wool method and the viral genomes were quantified using digital droplet PCR (ddPCR). All river water samples (15/15) were positive for the genomes of HAdVs, HAdV41, NoV GGI and NoV GGII. Concentrations of HAdVs, HAdV41 and NoV GII genomes were similar and HAdV41 represented 80% of HAdVs. Infectious HAdVs were quantified in these samples using an integrated cell culture-quantitative PCR method (ICC-qPCR); they were detected in 93% (14/15) and quantified in 53% (8/15) of the samples. Thus, infectious HAdVs represented 0.3 to 12.2% of total HAdV particles detected by ddPCR. Infectious HAdV41 particles were found in 73% (11/15) of the samples. This common presence of pathogenic enteric viruses underlines the impact of wastewater discharge on quality of surface waters and may constitute a threat for human health. The relative abundance of genome of HAdV41 underlines the need for studies focusing on the specific detection of its infectious forms along water cycle.