PRODUCTIVE INFECTION OF NORMAL CD40-ACTIVATED HUMAN B-LYMPHOCYTES BY HIV-1
PRODUCTIVE INFECTION OF NORMAL CD40-ACTIVATED HUMAN B-LYMPHOCYTES BY HIV-1
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DOI：
10.1097/00002030-199411000-00004
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发表时间：
1994-11-01
期刊：
影响因子：
3.8
通讯作者：
DARVEAU, A
中科院分区：
文献类型：
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作者：
POULIN, L;PAQUETTE, N;DARVEAU, A
目的：抗原驱动的B细胞增殖和成熟发生在淋巴组织中存在的生发中心。该过程高度依赖于B和T淋巴细胞之间的功能相互作用。存在于B细胞上的CD 40的体外活化模拟B细胞-T细胞相互作用并允许正常EB病毒（EBV）阴性B淋巴细胞增殖。在HIV-1血清阳性个体中，B细胞在通过生殖中心迁移时暴露于游离病毒颗粒和受感染的T淋巴细胞。HIV-1病毒暴露对CD 40活化的B淋巴细胞的影响因此被检查。方法：新鲜分离的B淋巴细胞体外培养，通过活化CD 40。在B淋巴细胞暴露于HIV-1后，监测B细胞增殖、HIV-1感染性和病毒产生。此外，HIV介导的感染B细胞和未感染的CD 4 + T淋巴细胞之间的融合进行了评估，在共培养assay.Results：EBV阴性，CD 40激活的人B淋巴细胞直接感染HIV-1。感染显著降低了它们的增殖率。在B细胞培养上清液中检测到病毒产生。许多融合事件表明，HIV-1感染的B淋巴细胞可以传播到T淋巴细胞以下HIV-1介导的这两种细胞type.Conclusion融合：鉴于B细胞-T细胞的相互作用在维持功能性免疫系统的重要性，B淋巴细胞发育的中断可能有直接的影响艾滋病进展的过程中。
Objective: Antigen-driven B-cell proliferation and maturation occur in germinal centres present in lymphoid tissues. This process is highly dependent on functional interactions between B and T lymphocytes. In vitro activation of CD40 present on B cells mimics B cell-T cell interactions and allows the proliferation of normal Epstein-Barr virus (EBV)-negative B lymphocytes. In HIV-1-seropositive individuals, B cells become exposed to free viral particles and to infected T lymphocytes while migrating through germinal centres. The effect of HIV-1 viral exposure on CD40-activated B lymphocytes was therefore examined.Methods: Freshly isolated B lymphocytes were cultured in vitro through activation of CD40. B-cell proliferation, HIV-1 infectivity and viral production were monitored following B-lymphocyte exposure to HIV-1. In addition, HIV-mediated fusion between infected B cells and uninfected CD4+ T lymphocytes was assessed in a coculture assay.Results: EBV-negative, CD40-activated human B lymphocytes were directly infected by HIV-1. The infection significantly reduced their proliferation rate. Viral production was detected in B-cell culture supernatant. Numerous fusion events indicated that HIV-1 infection of B lymphocytes could spread to T lymphocytes following HIV-1-mediated fusion of these two cell types.Conclusion: In view of the importance of B cell-T cell interactions in the maintenance of a functional immune system, disruption of B-lymphocyte development could have direct implications on the course of AIDS progression.