Processing of a precursor of 72-kilodalton type IV collagenase/gelatinase A by a recombinant membrane-type 1 matrix metalloproteinase.
Processing of a precursor of 72-kilodalton type IV collagenase/gelatinase A by a recombinant membrane-type 1 matrix metalloproteinase.
复制标题
通过重组膜 1 型基质金属蛋白酶处理 72 千道尔顿 IV 型胶原酶/明胶酶 A 的前体。
作者：
T. Kinoshita;Hiroshi Sato;T. Takino;M. Itoh;T. Akizawa;M. Seiki
膜型1基质金属蛋白酶是与蛋白水解激活的明胶酶A作为重组融合蛋白表达在大肠杆菌。重组酶以序列特异性方式切割明胶酶A的前肽序列。具有Asn（66）-Leu到Ile-Val的取代的突变体明胶酶A根本不被加工。金属蛋白酶组织抑制剂2或BB-94可阻断该过程，而金属蛋白酶组织抑制剂1则不阻断该过程。因此，膜型1基质金属蛋白酶是明胶酶A的直接激活剂，而不需要额外的蛋白酶。
Membrane-type 1 matrix metalloproteinase that is associated with the proteolytic activation of progelatinase A was expressed as a recombinant fusion protein in Escherichia coli. The recombinant enzyme cleaved the propeptide sequence of gelatinase A in a sequence-specific manner. A mutant progelatinase A that has a substitution of Asn(66)-Leu to Ile-Val was not processed at all. The processing was blocked by tissue inhibitor of metalloproteinases-2 or BB-94 but not by tissue inhibitor of metalloproteinases-1. Thus, membrane-type 1 matrix metalloproteinase is a direct activator of progelatinase A without requiring additional proteases.
癌症中细胞粘附蛋白的蛋白酶。
影响因子：
14.5
作者：
Monsky,WL;Chen,WT
通讯作者：
Chen,WT
DOI：
--
发表时间：
1993-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
A. Strongin;B. Marmer;G. A. Grant;G. Goldberg
通讯作者：
A. Strongin;B. Marmer;G. A. Grant;G. Goldberg