Monoclonal antibodies against HLA-DR antigens replace T helper cells in activation of B lymphocytes.
Monoclonal antibodies against HLA-DR antigens replace T helper cells in activation of B lymphocytes.
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针对 HLA-DR 抗原的单克隆抗体在 B 淋巴细胞的激活中替代 T 辅助细胞。
DOI：
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发表时间：
1983
影响因子：
11.1
通讯作者：
K. Guy
中科院分区：
文献类型：
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作者：
R. Palacios;O. Martínez;K. Guy
在商陆有丝分裂原 (PWM) 存在但不存在的情况下，针对 HLA-DR 抗原 147 和 164 的单克隆抗体有助于高度纯化的 B 淋巴细胞增殖并成熟为 Ig 分泌细胞。相反，抗DR抗体231和UCHT1单克隆抗人T细胞抗体（均与147和164抗DR抗体同种型）均未表现出对B细胞的任何辅助活性。用 PWM 脉冲并随后在抗 DR 抗体 147 或 164 存在下培养的 B 细胞增殖并分泌 Ig，而首先用抗体 147 或 164 脉冲然后用 PWM 孵育的 B 细胞则不会。单独的 PWM 不会在纯化的 B 细胞上诱导任何这些反应。此外，抗体 147 和 164 显着增加了从 WT51 Epstein-Barr 病毒转化的 B 细胞系获得的 Ig 分泌细胞的数量。抗DR抗体231抑制由抗DR抗体164介导的WT51细胞的辅助活性。最后，抗DR抗体147和164辅助来自无胸腺nu/nu小鼠（其携带I-E编码产物）的脾脏的B细胞产生针对绵羊红细胞的IgM抗体。然而，这些抗体对小鼠 B 细胞没有影响，因为小鼠 B 细胞表面不表达 I-E 编码的 Ia 抗原。总的来说，这些发现表明，B 淋巴细胞上的抗 DR 抗体 147 和 164 检测到的结构充当 T 细胞衍生的辅助信号的受体/转导器。
In the presence but not in the absence of pokeweed mitogen (PWM), monoclonal antibodies against HLA-DR antigens 147 and 164 helped highly purified B lymphocytes to proliferate and mature to Ig-secreting cells. In contrast, neither anti-DR antibody 231 nor the UCHT1 monoclonal anti-human T cell antibody (both of the same isotype as the 147 and 164 anti-DR antibodies) exhibited any helper activity on B cells. B cells pulsed with PWM and subsequently cultured in the presence of anti-DR antibody 147 or 164 proliferated and secreted Ig, whereas B cells that first were pulsed with antibody 147 or 164 and then incubated with PWM did not. PWM alone did not induce any of these responses on purified B cells. Also, antibodies 147 and 164 significantly increased the number of Ig-secreting cells obtained from the WT51 Epstein-Barr virus-transformed B-cell line. Anti-DR antibody 231 inhibited the helper activity on WT51 cells mediated by anti-DR antibody 164. Finally, anti-DR antibodies 147 and 164 assisted B cells from the spleen of athymic nu/nu mice (which bear I-E-encoded products) to produce IgM antibodies against sheep erythrocytes. However, these antibodies had no effect on mouse B cells which do not express on their surface I-E-encoded Ia antigens. Taken collectively, these findings suggest that the structures detected by anti-DR antibodies 147 and 164 on B lymphocytes function as acceptors/transducers of T-cell-derived helper signals.