Changes in photoperiod alter Glut4 expression in skeletal muscle of C57BL/6J mice.
Changes in photoperiod alter Glut4 expression in skeletal muscle of C57BL/6J mice.
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光周期的变化会改变 C57BL/6J 小鼠骨骼肌中 Glut4 的表达。
DOI：
10.1016/j.bbrc.2017.02.023
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发表时间：
2017
影响因子：
3.1
通讯作者：
Yasuo S
中科院分区：
文献类型：
--
作者：
Tashiro A;Shibata S;Takai Y;Uchiwa T;Furuse M;Yasuo S
光周期的季节性变化影响小鼠的体重和新陈代谢。在这里，我们研究了光周期变化对 C57BL/6J 小鼠骨骼肌和脂肪组织中葡萄糖转运蛋白基因表达的影响。暴露于短日照（SD）的小鼠腓肠肌中 Glut4 表达低于暴露于长日照（LD）的小鼠，并伴随着 GLUT4 蛋白水平的变化。尽管SD下小鼠比目鱼肌中的Glut4表达高于LD下，但GLUT4蛋白水平保持不变。为了确认光周期诱导的 Glut4 表达变化的功能意义，我们检查了胰岛素敏感性的变化。在SD下注射胰岛素后血糖水平仍然很高，这表明暴露于SD的小鼠对胰岛素的敏感性低于暴露于LD的小鼠。我们还试图阐明光周期变化后小鼠体内 Glut4 表达与体力活动之间的关系。通过红外光束传感器检测到的运动活动在 SD 下低于 LD 下。然而，当我们使用跑轮促进自愿活动时，两组小鼠的轮子旋转相似。尽管由于跑轮而增强了体力活动水平，但腓肠肌中的 Glut4 表达保持不变。因此，光周期的变化改变了小鼠骨骼肌中Glut4的表达，随后GLUT4蛋白水平和胰岛素敏感性发生变化。这些影响可能与体力活动无关。
Seasonal changes in photoperiod influence body weight and metabolism in mice. Here, we examined the effect of changes in photoperiod on the expression of glucose transporter genes in the skeletal muscle and adipose tissue of C57BL/6J mice.Glut4expression was lower in the gastrocnemius muscle of mice exposed to a short-duration day (SD) than those to a long-duration day (LD), with accompanying changes in GLUT4 protein levels. AlthoughGlut4expression in the mouse soleus muscle was higher under SD than under LD, GLUT4 protein levels remained unchanged. To confirm the functional significance of photoperiod-induced changes inGlut4expression, we checked for variations in insulin sensitivity. Blood glucose levels after insulin injection remained high under SD, suggesting that the mice exposed to SD showed lower sensitivity to insulin than those exposed to LD. We also attempted to clarify the relationship betweenGlut4expression and physical activity in the mice following changes in photoperiod. Locomotor activity, as detected via infrared beam sensor, was lower under SD than under LD. However, when we facilitated voluntary activity by using running wheels, the rotation of wheels was similar for both groups of mice. Although physical activity levels were enhanced due to running wheels,Glut4expression in the gastrocnemius muscle remained unchanged. Thus, variations in photoperiod alteredGlut4expression in the mouse skeletal muscle, with subsequent changes in GLUT4 protein levels and insulin sensitivity; these effects might be independent of physical activity.
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