CONVERSION OF LOGARITHMIC CHANNEL NUMBERS INTO RELATIVE LINEAR FLUORESCENCE INTENSITY
CONVERSION OF LOGARITHMIC CHANNEL NUMBERS INTO RELATIVE LINEAR FLUORESCENCE INTENSITY
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DOI：
10.1002/cyto.990090605
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发表时间：
1988-11-01
期刊：
影响因子：
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通讯作者：
GIORGI, JV
中科院分区：
文献类型：
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作者：
SCHMID, I;SCHMID, P;GIORGI, JV
我们描述了一种简单、可重复且普遍适用的方法，通过使用提供不同强度的多个峰的荧光样本来评估对数放大器的性能。多个峰之间的通道差被用来评价流式细胞仪上荧光信号放大器的对数行为。可以创建校准曲线来校正通道数与真实对数行为的偏差，然后将数据转换为相对线性强度。通过使用这些线性荧光强度，我们比较了不同的抗HIV-1(人类免疫缺陷病毒1型)多肽抗血清抑制HIV-1与CD4阳性T细胞系CEM结合的能力。这种校准程序的广泛应用是可以预见的，该方法对于随着时间的推移监测仪器的性能是有价值的。
We describe a simple, reproducible, and generally applicable method to assess the performance of log amplifiers by using a fluorescent sample that provides multiple peaks of different intensities. The channel differences between multiple peaks are used to evaluate the logarithmic behavior of the fluorescence signal amplifier on the flow cytometer. A calibration curve can be created to correct the channel numbers for deviations from true logarithmic behavior and then convert data into relative linear intensities. By using these linear fluorescent intensities, we compared the capacity of different antisera against HIV-1 (human immunodeficiency virus type 1) peptides to inhibit the binding of HIV-1 to CEM, a CD4-positive T-cell line. A wide range of applications for this calibration procedure can be envisioned and the method is valuable for monitoring instrument performance over time.