Measurement of in vivo cholesterol synthesis from 2H2O: a rapid procedure for the isolation, combustion, and isotopic assay of erythrocyte cholesterol.
Measurement of in vivo cholesterol synthesis from 2H2O: a rapid procedure for the isolation, combustion, and isotopic assay of erythrocyte cholesterol.
复制标题
2H2O 体内胆固醇合成的测量：红细胞胆固醇的分离、燃烧和同位素测定的快速程序。
作者：
W. Wong;D. Hachey;A. Feste;J. Leggitt;L. L. Clarke;W. Pond;P. Klein
建立了一种快速制备级红细胞游离胆固醇的纯化方法，用于测定2H2O在体内胆固醇合成。所获得的胆固醇的量和纯度适合于燃烧、所形成的水的锌还原以及通过气体同位素比质谱法测定氘同位素含量。通过对接受不添加和添加膳食胆固醇的膳食的仔猪进行测量来说明检测和定量一系列胆固醇合成速率的能力。
A rapid preparative scale purification of erythrocyte free cholesterol has been developed for measurements of in vivo cholesterol synthesis from 2H2O. The quantity and purity of cholesterol obtained is suitable for combustion, zinc reduction of the water formed, and determination of deuterium isotopic content by gas isotope ratio mass spectrometry. The ability to detect and to quantitate a range of cholesterol synthesis rates is illustrated by measurements on young pigs receiving diets without and with added dietary cholesterol.
影响因子：
6.5
作者：
J. Dietschy;D. Spady
通讯作者：
J. Dietschy;D. Spady