Identification of human hepatoma-defined cell surface molecules.
Identification of human hepatoma-defined cell surface molecules.
复制标题
人肝癌细胞表面分子的鉴定。
作者：
Moriarity,DM;Fox,N;Aden,DP;Hoyer,JR;Knowles,BB
用人肝癌Hep G2细胞免疫BALB/c小鼠，取脾细胞与SP2/0-Ag 14小鼠骨髓瘤细胞融合。本文报道了两株识别肝癌细胞表面抗原决定簇（Hag-1、Hag-2）的单克隆抗体。通过十二烷基硫酸钠（SDS）凝胶电泳分析免疫沉淀物显示，Hag-1抗原决定簇产生于分子量为115 kD的糖蛋白上，并且第二抗体免疫沉淀一组分子量为230 kD、79 kD、23 kD和20 kD的来自人肝癌细胞的表面蛋白。这些抗原决定簇存在于来源于其他人类肿瘤的细胞系上，因此两种抗体都不是肝癌特异性的;与人类结直肠癌和一些乳腺癌细胞系的交叉反应性是显著的。使用间接免疫荧光法在冷冻切片上检测到Hag-1，而Hag-2在所有三个肝活检测试之一。在人肾切片中检测到这两种抗原，Hag-2定位于近端小管。
BALB/c mouse splenocytes from mice immunized with cells of the human hepatoma line Hep G2 were fused with SP2/0-Ag 14 mouse myeloma cells. Two monoclonal antibodies recognizing antigenic determinants (Hag-1, Hag-2) of hepatoma cell surface molecules were investigated. Analysis of immunoprecipitates by sodium dodecyl sulfate (SDS) gel electrophoresis revealed that the Hag-1 antigenic determinant is born on a 115, kD MW glycoprotein, and that the second antibody immunoprecipitates a group of surface proteins with MW of 230 kD, 79 kD, 23 kD, and 20 kD from human hepatoma cells. These antigenic determinants are present on cell lines derived from other human tumors, thus neither of the antibodies is hepatoma-specific; cross-reactivity with human colorectal carcinoma and some mammary carcinoma cell lines is notable. Using indirect immunofluorescence on frozen sections Hag-1 was detected in one of three liver biopsies tested whereas Hag-2 was demonstrated in all three. Both antigens were detected in sections of human kidney with Hag-2 localized to the proximal tubules.