Isolation and characterization of bacterial strains that have high ability to degrade 1,4-dioxane as a sole carbon and energy source
Isolation and characterization of bacterial strains that have high ability to degrade 1,4-dioxane as a sole carbon and energy source
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DOI：
10.1007/s10532-012-9614-1
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发表时间：
2013-09
期刊：
影响因子：
3.6
通讯作者：
K. Sei;Keiko Miyagaki;Takashi Kakinoki;Kunihiro Fukugasako;D. Inoue;M. Ike
中科院分区：
文献类型：
--
作者：
K. Sei;Keiko Miyagaki;Takashi Kakinoki;Kunihiro Fukugasako;D. Inoue;M. Ike
从某化工厂排水区的土壤中分离到4株具有较高降解能力的代谢型1，4-二氧六环降解菌，命名为D1、D 6、D11和D17。菌株D 6、D11和D17属于革兰氏阳性放线菌，与先前报道的代谢1，4-二氧六环降解菌相似，而菌株D1属于革兰氏阴性Afipiasp。分离的菌株可以利用各种碳源，包括环醚，特别是那些在2位的碳被甲基或羰基修饰。1，4-二氧六环的细胞产率相对较低（0.179-0.223 mg-蛋白质（mg-1，4-二氧六环）-1），这可能是由于C-O键分裂需要能量。分离菌株的1，4-二氧六环特异降解率是对照菌株的2.6-13倍（0.052-0.263 mg-1，4-二氧六环（mg-蛋白质）−1h−1）和2.3-7.8倍下半饱和常数（20.6-69.8 mg L-1），比迄今为止报道的最有效的1，4-二氧六环降解细菌Pseudonocardia dioxanivoransCB 1190，表明对1，4-二氧六环降解具有高活性和亲和力。菌株D1和D 6具有诱导型1，4-二氧六环降解酶，而菌株D11和D17具有组成型1，4-二氧六环降解酶。Afipiasp降解1，4-二氧六环（100 mg L−1）。D1不受高达3，000 mg L− 1乙二醇共存的影响。初始pH值、培养温度和NaCl浓度对4株菌降解1，4-二氧六环的影响表明，它们在较宽的条件范围内都能降解1，4-二氧六环，对工业废水处理具有一定的适应性和适用性。
Four novel metabolic 1,4-dioxane degrading bacteria possessing high ability to degrade 1,4-dioxane (designated strains D1, D6, D11 and D17) were isolated from soil in the drainage area of a chemical factory. Strains D6, D11 and D17 were allocated to Gram-positive actinomycetes, similar to previously reported metabolic 1,4-dioxane degrading bacteria, whereas strain D1 was allocated to Gram-negativeAfipiasp. The isolated strains could utilize a variety of carbon sources, including cyclic ethers, especially those with carbons at position 2 that were modified with methyl- or carbonyl-groups. The cell yields on 1,4-dioxane were relatively low (0.179–0.223 mg-protein (mg-1,4-dioxane)−1), which was likely due to requiring energy for C–O bond fission. The isolated strains showed 2.6–13 times higher specific 1,4-dioxane degradation rates (0.052–0.263 mg-1,4-dioxane (mg-protein)−1h−1) and 2.3–7.8 fold lower half saturation constants (20.6–69.8 mg L−1) than the most effective 1,4-dioxane degrading bacterium reported to date,Pseudonocardia dioxanivoransCB1190, suggesting high activity and affinity toward 1,4-dioxane degradation. Strains D1 and D6 possessed inducible 1,4-dioxane degrading enzymes, whereas strains D11 and D17 possessed constitutive ones. 1,4-Dioxane degradation (100 mg L−1) byAfipiasp. D1 was not affected by the co-existence of up to 3,000 mg L−1of ethylene glycol. The effects of initial pH, incubation temperature and NaCl concentration on 1,4-dioxane degradation by the four strains revealed that they could degrade 1,4-dioxane under a relatively wide range of conditions, suggesting that they have a certain adaptability and applicability for industrial wastewater treatment.