Analysis of linear B-cell epitopes of the nucleoprotein of Ebola virus that distinguish Ebola virus subtypes
Analysis of linear B-cell epitopes of the nucleoprotein of Ebola virus that distinguish Ebola virus subtypes
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DOI：
10.1128/cdli.10.1.83-87.2003
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发表时间：
2003-01-01
期刊：
影响因子：
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通讯作者：
Morikawa, S
中科院分区：
文献类型：
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作者：
Niikura, M;Ikegami, T;Morikawa, S
埃博拉病毒由四种基因上可区分的亚型组成。我们开发了针对埃博拉病毒扎伊尔亚型核蛋白 (NP) 的单克隆抗体 (MAb)，并分析了它们与雷斯顿亚型和苏丹亚型的交叉反应性。我们进一步确定了这些单克隆抗体识别的表位。三种 MAb 与三种主要亚型反应并识别 C 末端包含 110 个氨基酸 (aa) 的片段。在 Pepscan 分析中，它们没有表现出对任何 10 个氨基酸短肽的特异性反应，这表明这些 MAb 识别位于该区域内的构象表位。六种 MAb 识别对应于 NP 氨基酸 361 至 461 的片段。这六种 MAb 中的五种在 Pepscan 分析中显示出特异性反应性，并在两个区域（aa 424 至 430 和 aa 451 至 455）中鉴定出表位。识别前一表位区域的三种 MAb 与所有三种亚型发生交叉反应，识别相同表位区域的一种是扎伊尔特异性的。一种识别后一表位区域的单克隆抗体与扎伊尔和苏丹亚型有反应，但与雷斯顿亚型不反应。这些结果表明，埃博拉病毒 NP 具有至少两个线性表位区域，并且单克隆抗体对表位的识别甚至在同一表位区域内也可能有所不同。这些显示出不同亚型特异性的单克隆抗体可能是开发免疫系统以识别埃博拉病毒亚型的有用试剂。
Ebola virus consists of four genetically distinguishable subtypes. We developed monoclonal antibodies (MAbs) to the nucleoprotein (NP) of Ebola virus Zaire subtype and analyzed their cross-reactivities to the Reston and Sudan subtypes. We further determined the epitopes recognized by these MAbs. Three MAbs reacted with the three major subtypes and recognized a fragment containing 110 amino acids (aa) at the C-terminal extremity. They did not show specific reactivities to any 10-aa short peptides in Pepscan analyses, suggesting that these MAbs recognize conformational epitope(s) located within this region. Six MAbs recognized a fragment corresponding to aa 361 to 461 of the NP. Five of these six MAbs showed specific reactivities in Pepscan analyses, and the epitopes were identified in two regions, aa 424 to 430 and aa 451 to 455. Three MAbs that recognized the former epitope region cross-reacted with all three subtypes, and one that recognized the same epitope region was Zaire specific. One MAb, which recognized the latter epitope region, was reactive with Zaire and Sudan subtypes but not with the Reston subtype. These results suggest that Ebola virus NP has at least two linear epitope regions and that the recognition of the epitope by MAbs can vary even within the same epitope region. These MAbs showing different subtype specificities might be useful reagents for developing an immunological system to identify Ebola virus subtypes.