Delivery of fluorescent probes using iron oxide particles as carriers enables in-vivo labeling of migrating neural precursors for magnetic resonance imaging and optical imaging
Delivery of fluorescent probes using iron oxide particles as carriers enables in-vivo labeling of migrating neural precursors for magnetic resonance imaging and optical imaging
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DOI：
10.1117/1.2800294
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发表时间：
2007-09-01
影响因子：
3.5
通讯作者：
Koretsky, Alan P.
中科院分区：
文献类型：
--
作者：
Sumner, James P.;Conroy, Richard;Koretsky, Alan P.
氧化铁颗粒正在成为磁共振成像(MRI)细胞跟踪研究的重要造影剂。将带有颗粒的荧光指示剂同时传送到单个细胞提供了将光学图像和核磁共振相关联的可能性。在这项工作中，微米级的氧化铁颗粒(MPIO)可以作为载体向培养的细胞和体内迁移的神经前体细胞输送荧光探针。由于使用了荧光探针，MRI可以跟踪迁移的祖细胞，并很容易通过组织学进行识别。这些数据表明，使用MPIO来传递荧光探针，应该可以将MRI和光学成像结合起来，用于体内细胞跟踪。(C)2007年，光学仪器工程师学会。
Iron oxide particles are becoming an important contrast agent for magnetic resonance imaging (MRI) cell tracking studies. Simultaneous delivery of fluorescence indicators with the particles to individual cells offers the possibility of correlating optical images and MRI. In this work, it is demonstrated that micron-sized iron oxide particles (MPIOs) can be used as a carrier to deliver fluorescent probes to cells in culture as well as to migrating neural progenitors in vivo. Migrating progenitors were tracked with MRI and easily identified by histology because of the fluorescent probe. These data suggest that using MPIOs to deliver fluorescent probes should make it possible to combine MRI and optical imaging for in vivo cell tracking. (c) 2007 Society of Photo-Optical Instrumentation Engineers.