DYNAMICS OF CEREBRAL METABOLISM DURING MODERATE HYPERCAPNIA 1
DYNAMICS OF CEREBRAL METABOLISM DURING MODERATE HYPERCAPNIA 1
复制标题
中度高碳酸血症期间脑代谢的动力学 1
DOI：
10.1111/j.1471-4159.1975.tb07664.x
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发表时间：
1975
影响因子：
4.7
通讯作者：
O. Reinmuth
中科院分区：
文献类型：
--
作者：
K. Kogure;R. Busto;P. Scheinberg;O. Reinmuth
高碳酸血症对成年大鼠脑能代谢动力学的影响，采用LOWRY等（1964）的封闭系统方法进行了评估。Paco2为61 torr的中度高碳酸血症持续20分钟导致细胞内脑酸中毒（7.07‐6.97）。高碳酸血症期间，组织葡萄糖含量升高，磷酸肌酸、ADP、丙酮酸和乳酸含量升高，乳酸/丙酮酸比值降低。ATP/ADP比值由7.7增加到9.0；胞质NADH/NAD +比值由2.06 × 10‐3降至1.49 × 10‐3。能量收费没有变化。磷酸肌酸的周转率从3.84 mmol/kg/min增加到4.62 mmol/kg/min，而ATP、葡萄糖和糖原的周转率分别从1.98降至1.86、6.24降至4.80和3.96降至2.94 mmol/kg/min。总高能磷酸盐利用率从30.6 mmol/kg/min降至25.4 mmol/kg/min，且高碳酸血症时斩首后脑电图持续时间比低碳酸血症时更长。这些结果表明，中度高碳酸血症降低了大脑能量代谢的整体动力学活性。稳定的能量电荷表明，高能量磷酸盐利用率的降低与ATP产量的降低成比例地平衡。
The effects of hypercapnia on the kinetics of cerebral energy metabolism were evaluated in adult rats by the closed system method of LOWRY et al. (1964). Moderate hypercapnia with a Paco2 of 61 torr sustained for 20 min resulted in intracellular brain acidosis (7.07‐6.97). During hypercapnia the tissue content of glucose increased whereas phosphocreatine, ADP, pyruvate and lactate contents, and the lactate/pyruvate ratio decreased. The ATP/ADP ratio increased from 7.7 to 9.0; the cytoplasmic NADH/NAD + ratio decreased from 2.06 × 10‐3 to 1.49 × 10‐3. There was no change in Energy Charge. Turnover rate of phosphocreatine increased from 3.84 to 4.62 mmol/kg/min, but the turnover rates of ATP, glucose and glycogen were reduced (from 1.98 to 1.86, 6.24 to 4.80, and 3.96 to 2.94 mmol/kg/min, respectively). The utilization rate of total high energy phosphate decreased from 30.6 to 25.4 mmol/kg/min while the post‐decapitation EEG during hypercapnia persisted longer than during normocapnia. These results indicate that moderate hypercapnia reduces the overall kinetic activity of cerebral energy metabolism. The steady Energy Charge suggests that the reduction in the rate of high energy phosphate use is proportionally balanced by a lowered production rate of ATP.