Controlled formation and stabilization of nanosized colloidal suspensions by combination of soy protein and biosurfactant stevioside as stabilizers
Controlled formation and stabilization of nanosized colloidal suspensions by combination of soy protein and biosurfactant stevioside as stabilizers
复制标题
通过大豆蛋白和生物表面活性剂甜菊苷作为稳定剂的组合来控制纳米胶体悬浮液的形成和稳定
DOI：
10.1016/j.foodhyd.2015.07.005
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发表时间：
2016
影响因子：
10.7
通讯作者：
Wang Li-Juan
中科院分区：
文献类型：
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作者：
Wan Zhi-Li;Wang Li-Ying;Yang Xiao-Quan;Wang Jin-Mei;Wang Li-Juan
在这项工作中，我们评估了变性大豆蛋白（SPI）和生物表面活性剂甜菊苷（STE）的新型组合作为高效新型稳定剂的潜力，用于使用白藜芦醇（RES）作为模型化合物生产稳定的纳米混悬剂。采用纳米沉淀-超声法制备RES纳米混悬液(RESN)。在低于 STE 的 CMC (0.25–0.5%) 时，SPI-STE 混合物稳定的 RESN 表现出出色的物理化学特性，例如小粒径（低于 200 nm）和高收率/包封效率（高于 97%），这应归因于 SPI-STE 络合导致混合物具有更高的表面活性，从而在颗粒表面更快吸附，从而有助于粒径的减小。同时，小分子STE可以作为填料来填充SPI留下的缺陷，从而在颗粒表面提供更完整和致密的覆盖。因此，RESN表现出显着的储存稳定性，即使在没有任何保护剂的情况下，直接冷冻干燥后也能保持良好的再分散性。然而，在高 STE 浓度（1-2%，高于其 CMC）下，由于混合物表面活性降低和 STE 胶束的存在，RESN 的形成受到阻碍，这可能导致颗粒表面受到不完全保护。结果，所得 RESN 变得非常不稳定，并且在储存过程中极易发生颗粒聚集。
In this work, we evaluated the potential of a novel combination of denatured soy protein (SPI) and biosurfactant stevioside (STE) as efficient and novel stabilizers for producing stable nanosuspensions using resveratrol (RES) as a model compound. The RES nanosuspensions (RESN) were prepared by the nanoprecipitation-ultrasonication method. Below the CMC of STE (0.25–0.5%), the RESN stabilized by the SPI-STE mixtures exhibited outstanding physicochemical characteristics, such as small particle size (below 200 nm) and high yield/entrapment efficiency (above 97%), which should be ascribed to the higher surface activity of mixtures due to SPI-STE complexation and hence faster adsorption on particle surfaces, thus contributing to the decrease in particle size. Meanwhile, the small molecule STE could function as a filler to fill the defects left by SPI, thus providing a more complete and compact coverage on particle surface. Accordingly, the RESN exhibited a remarkable storage stability and could maintain good redispersibility after direct freeze-drying even without any protectants. Nevertheless, at high STE concentrations (1–2%, above its CMC), the formation of RESN was hindered due to the decreased surface activity of mixtures and the presence of STE micelles, which could cause particle surfaces incompletely protected. As a result, the resultant RESN became very unstable and highly susceptible to particle aggregation during storage.