Metagenomic natural product discovery in lichen provides evidence for a family of biosynthetic pathways in diverse symbioses
Metagenomic natural product discovery in lichen provides evidence for a family of biosynthetic pathways in diverse symbioses
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DOI：
10.1073/pnas.1305867110
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发表时间：
2013-08-13
影响因子：
11.1
通讯作者：
Andresson, Olafur S.
中科院分区：
文献类型：
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作者：
Kampa, Annette;Gagunashvili, Andrey N.;Andresson, Olafur S.
细菌是天然产物的主要来源，为化学和生物学研究提供了丰富的机会。虽然绝大多数已知的细菌代谢产物来自自由生活的生物体，但越来越多的证据支持广泛存在的共生体中的化学多产细菌。基于生物信息学预测，共生体培养，同位素富集和先进的分析策略被用来表征一个独特的聚酮，nosperin，从地衣相关的念珠藻属蓝藻。从30微克化合物中确定的nosperin的生物合成基因簇和结构，与以前只从与甲虫和海绵有关的非光合细菌中知道的pederin组有关。这种天然产物家族在如此高度不同的关联中的存在表明，一些细菌代谢产物可能特异于与真核生物的共生，并鼓励探索用于药物发现的其他共生体，以及更好地理解由复杂的细菌代谢产物介导的生态相互作用。
Bacteria are a major source of natural products that provide rich opportunities for both chemical and biological investigation. Although the vast majority of known bacterial metabolites derive from free-living organisms, increasing evidence supports the wide-spread existence of chemically prolific bacteria living in symbioses. A strategy based on bioinformatic prediction, symbiont cultivation, isotopic enrichment, and advanced analytics was used to characterize a unique polyketide, nosperin, from a lichen-associated Nostoc sp. cyanobacterium. The biosynthetic gene cluster and the structure of nosperin, determined from 30 mu g of compound, are related to those of the pederin group previously known only from nonphoto-synthetic bacteria associated with beetles and marine sponges. The presence of this natural product family in such highly dissimilar associations suggests that some bacterial metabolites may be specific to symbioses with eukaryotes and encourages exploration of other symbioses for drug discovery and better understanding of ecological interactions mediated by complex bacterial metabolites.