Detection and genome characterization of two novel papillomaviruses and a novel polyomavirus in tree shrew (Tupaia belangeri chinensis) in China
Detection and genome characterization of two novel papillomaviruses and a novel polyomavirus in tree shrew (Tupaia belangeri chinensis) in China
复制标题
中国树鼩（Tupaia belangeri chinensis）中两种新型乳头瘤病毒和一种新型多瘤病毒的检测和基因组特征
DOI：
10.1186/s12985-019-1141-9
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发表时间：
2019-03-18
期刊：
影响因子：
4.8
通讯作者：
Ge, Xing-Yi
中科院分区：
文献类型：
--
作者：
Liu, Ping;Qiu, Ye;Ge, Xing-Yi
研究背景乳头瘤病毒(PV)和多瘤病毒(PYV)感染包括人类在内的多种脊椎动物，引起从无症状感染到严重疾病的广泛后果。虽然树鼠的基因组特征与灵长类动物高度相似，但未检测到PV，仅在树鼠体内检测到一种PYV。方法对采自云南省中国地区的树鼠标本和器官标本进行病毒基因组分析和随机聚合酶链式反应检测PV和PYV的存在。结果在中国树鼠(Tupaia Belangeri Chinensis)中发现了两种新病毒和一种新的PYV，分别命名为树鼠乳头状瘤病毒1和2(TbelPV1和TbelPV2)和多瘤病毒1(TbelPyV1)。TbelPV1、TbelPV2和TbelPyV1的基因组大小分别为7410 、7526 和4982 。TbelPV1基因组包含7个开放阅读框架(ORF)，编码病毒蛋白E1、E2、E4、E6、E7、L1和L2；TbelPV2基因组包含6个ORF，编码病毒蛋白E1、E2、E6、E7、L1和L2；TbelPyV1基因组编码典型的PYV大小T抗原以及VP1、VP2和VP3衣壳蛋白。基因组比较和系统发育分析表明，TbelPV1和TbelPV2代表了乳头病毒科的2个新的PV属，而TbelPyV1代表了甲型多瘤病毒的一个新种。我们的流行病学研究表明，在口腔拭子和脾中都检测到TbelPV1和TbelPV2，而在口腔拭子和脾中检测到TbelPyV1。结论在树鼠体内发现了两种新的PVs(TbelPV1和TbelPV2)和一种新的PYV(TbelPyV)，并对其基因组进行了特征分析。TbelPV1、TbelPV2和TbelPyV1分别与人乳头瘤病毒63型、乳头虫乳头瘤病毒1型和人多瘤病毒9型具有最高的相似性。TbelPV1和TbelPV2只表现为口服嗜好，而TbelPyV1表现为口服和脾嗜好。
BackgroundPapillomaviruses (PVs) and polyomaviruses (PyVs) infect diverse vertebrates including human and cause a broad spectrum of outcomes from asymptomatic infection to severe disease. There has been no PV and only one PyV detected in tree shrews, though the genomic properties of tree shrews are highly similar to those of the primates.MethodsSwab and organ samples of tree shrews collected in the Yunnan Province of China, were tested by viral metagenomic analysis and random PCR to detect the presence of PVs and PyVs. By PCR amplification using specific primers, cloning, sequencing and assembling, genomes of two PVs and one PyV were identified in the samples.ResultsTwo novel PVs and a novel PyV, named tree shrew papillomavirus 1 and 2 (TbelPV1 and TbelPV2) and polyomavirus 1 (TbelPyV1) were characterized in the Chinese tree shrew (Tupaia belangeri chinensis). The genomes of TbelPV1, TbelPV2, and TbelPyV1 are 7410 bp, 7526 bp, and 4982 bp in size, respectively. The TbelPV1 genome contains 7 putative open-reading frames (ORFs) coding for viral proteins E1, E2, E4, E6, E7, L1, and L2; the TbelPV2 genome contains 6 ORFs coding for viral proteins E1, E2, E6, E7, L1, and L2; and the TbelPyV1 genome codes for the typical small and large T antigens of PyV, as well as the VP1, VP2, and VP3 capsid proteins. Genomic comparison and phylogenetic analysis indicated that TbelPV1 and TbelPV2 represented 2 novel PV genera ofPapillomaviridae, and TbelPyV1 represented a new species of genusAlphapolyomavirus. Our epidemiologic study indicated that TbelPV1 and TbelPV2 were both detected in oral swabs, while TbelPyV1 was detected in oral swabs and spleens.ConclusionTwo novel PVs (TbelPV1 and TbelPV2) and a novel PyV (TbelPyV) were discovered in tree shrews and their genomes were characterized. TbelPV1, TbelPV2, and TbelPyV1 have the highest similarity toHuman papillomavirus type 63,Ursus maritimus papillomavirus 1, andHuman polyomavirus 9, respectively. TbelPV1 and TbelPV2 only showed oral tropism, while TbelPyV1 showed oral and spleen tropism.