Are all caudates boneheads? Exploring lifestyle signals in the microanatomy of amphibian skull roofs
Are all caudates boneheads? Exploring lifestyle signals in the microanatomy of amphibian skull roofs
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尾核都是骨头吗？
DOI：
10.1093/icb/icad002
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发表时间：
2023
影响因子：
2.6
通讯作者：
Ebel, R.
中科院分区：
文献类型：
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作者：
Manning, S.;Nohomovich, M.;Olori, J.;Amson, E.;Ebel, R.
随着珊瑚礁的持续减少，表观遗传学和对环境变化的反应之间的关系值得研究。在无脊椎动物中，表观遗传标记几乎完全以基因体甲基化(GBM)的形式存在。在这里，我们测试了环境驱动的GBM变化是否会影响珊瑚礁建造中的基因表达可塑性。在我们的实验中，除了由于一天中的时间而导致的每日温度波动外，珊瑚碎片还受到了超过四周的加热或控制条件的影响。在实验接近尾声时，一些受热的珊瑚被切换到对照条件，看看它们的表达是否会恢复到受热状态，这表明可塑性降低。当采用转录组范围的方法时，我们发现治疗驱动的GBM变化和可塑性降低之间没有显著的相关性。关于日常表达的变化，我们发现有证据表明，一些基因在实验结束时降低了可塑性。然而，这与GBM的变化无关。最后，为了寻找塑性降低和GBM变化的细微尺度特征，我们确定了共同调节基因的模块，其中一个模块在热处理后显示出塑性降低。然而，在加热组和对照组中，该模块中的基因成员数与可塑性之间没有显著的相关性，而在切换组中，相关性可以忽略不计。根据这些结果，我们得出结论，虽然GBM可以随着时间的推移而改变，但这对基因没有影响。
As coral reefs continue to decline, the relationship between epigenetics and response to environmental change warrants investigation. In invertebrates, epigenetic markers are almost entirely in the form of gene body methylation (GBM). Here, we tested whether environmentally-driven changes in GBM impact gene expression plasticity in a reef-building coral. In our experiment, coral fragments were subjected to either heat or control conditions over four weeks, in addition to daily temperature fluctuations due to the time of day. Near the end of the experiment, some heated corals were switched to the control condition to see if their expression would revert remain in the heated state, indicating reduced plasticity. When taking a transcriptome-wide approach, we found no significant correlation between treatment-driven GBM change and reduced plasticity. In regards to daily expression changes, we found evidence that some genes had reduced plasticity at the end of the experiment. However, this was unrelated to GBM change. Finally, to look for fine-scale signatures of reduced plasticity and GBM change we identified modules of co-regulated genes, one of which showed reduced plasticity following heat treatment. However, there was no significant correlation between gene membership in this module and plasticity in the heat and control groups, and only a negligible correlation in the switched group. We conclude from these results that although GBM can change over time, this has no bearing on gene.