Myosin-cross-reactive antigens from four different lactic acid bacteria are fatty acid hydratases
Myosin-cross-reactive antigens from four different lactic acid bacteria are fatty acid hydratases
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来自四种不同乳酸菌的肌球蛋白交叉反应抗原是脂肪酸水合酶
DOI：
10.1007/s10529-012-1044-y
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发表时间：
2013-01-01
影响因子：
2.7
通讯作者：
Chen, Wei
中科院分区：
文献类型：
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作者：
Yang, Bo;Chen, Haiqin;Chen, Wei
67kda的肌球蛋白交叉反应性抗原（MCRA）是广泛存在于细菌中的MCRA蛋白家族的一员，预计具有脂肪酸异构酶功能。我们现在已经从四种LAB染色中表征了mcra的催化活性，包括鼠李糖乳杆菌lgg，L。plantarumST-III, L。嗜酸杆菌ncfm和动物双歧杆菌bb -12。克隆这些菌株的MCRA基因并在大肠杆菌中表达，利用气相色谱-质谱分析重组蛋白的功能和脂质谱。这4种MCRA蛋白催化了亚油酸和油酸转化为各自的10-羟基衍生物，表明MCRA蛋白催化了共轭亚油酸生产的第一步。这是来自ml的第一份MCRA报告。鼠李糖具有这样的催化功能。
The 67 kDa myosin-cross-reactive antigen (MCRA) is a member of the MCRA family of proteins present in a wide range of bacteria and was predicted to have fatty acid isomerase function. We have now characterised the catalytic activity of MCRAs from four LAB stains, includingLactobacillus rhamnosusLGG,L. plantarumST-III,L. acidophilusNCFM andBifidobacterium animalissubsp.lactisBB-12. MCRA genes from these strains were cloned and expressed inEscherichia coli,and the recombinant protein function was analysed with lipid profiles by GC–MS. The four MCRAs catalysed the conversion of linoleic acid and oleic acid to their respective 10-hydroxy derivatives, which suggests that MCRA proteins catalyse the first step in conjugated linoleic acid production. This is the first report of MCRA fromL. rhamnosuswith such catalytic function.