Probiotic Lactobacillus rhamnosus GG prevents alveolar bone loss in a mouse model of experimental periodontitis
Probiotic Lactobacillus rhamnosus GG prevents alveolar bone loss in a mouse model of experimental periodontitis
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DOI：
10.1111/jcpe.12838
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发表时间：
2018-02-01
影响因子：
6.7
通讯作者：
Bartold, Peter M.
中科院分区：
文献类型：
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作者：
Gatej, Simona M.;Marino, Victor;Bartold, Peter M.
目的研究鼠李糖乳杆菌GG （LGG）对实验性牙周炎（PD）小鼠体内模型骨质流失和局部及全身炎症的影响。材料与方法采用口腔接种牙龈卟啉单胞菌和核梭杆菌的方法，连续44d诱导小鼠PD。在疾病诱导前和诱导过程中，通过口服接种或灌胃给予益生菌LGG。并测定了LGG对接种菌的抑菌活性。利用体内显微计算机断层扫描和组织学分析评估牙槽骨水平和牙龈组织变化。采用多重测定法测定小鼠血清IL-8同源物水平。结果益生菌灌胃和口服接种治疗前后骨质流失明显减少(p
AimThis study investigated the role of Lactobacillus rhamnosus GG (LGG) on bone loss and local and systemic inflammation in an in vivo mouse model of experimental periodontitis (PD).Materials and MethodsExperimental PD was induced in mice by oral inoculation with Porphyromonas gingivalis and Fusobacterium nucleatum over a period of 44days. The probiotic LGG was administered via oral inoculation or oral gavage prior to, and during disease induction. The antimicrobial activity of LGG on the inoculum was also tested. Alveolar bone levels and gingival tissue changes were assessed using in vivo microcomputed tomography and histological analysis. Serum levels of mouse homologues for IL-8 were measured using multiplex assays.ResultsPre-treatment with probiotics either via oral gavage or via oral inoculation significantly reduced bone loss (p