Polaromonas naphthalenivorans sp nov., a naphthalene-degrading bacterium from naphthalene-contaminated sediment
Polaromonas naphthalenivorans sp nov., a naphthalene-degrading bacterium from naphthalene-contaminated sediment
复制标题
DOI：
10.1099/ijs.0.02636-0
复制
发表时间：
2004-01-01
影响因子：
2.8
通讯作者：
Madsen, EL
中科院分区：
文献类型：
--
作者：
Jeon, CO;Park, W;Madsen, EL
从煤焦油污染的淡水沉积物中分离到以萘为唯一碳源和能量源的菌株CJ2(T)。菌株CJ2(T)革兰氏反应为阴性。细胞不形成孢子，不运动的球菌（无鞭毛）。该分离物被发现是一种能够利用葡萄糖和其他单糖的需氧异养生物。生长在4 ~ 25℃（最适20℃）和pH 6.0 ~ 9.0（最适pH 7.0 ~ 7.5）之间。基因组DNA中G + C含量为61.5 mol%，醌类主要为泛醌-8。菌株CJ2(T)的肽聚糖属a1 - γ中二氨基戊酸。菌株CJ2(T)的主要脂肪酸为16:1omega7c（67.0%）、16:0（19.6%）、18:1 omega7c（7.9%）和10:0 3-OH（2.5%）。极性脂质为磷脂酰乙醇胺、磷脂酰甘油和二磷脂酰甘油。未检出霉菌酸和糖脂。比较16S rDNA分析表明，菌株CJ2(T)与Comamonadaceae有亲缘关系，与Polaromonas vacuolata 34-p(T)亲缘关系最近，相似性为97.1%。根据生理和分子特性，鉴定该萘降解菌为Polaromonas naphthalenivorans sp. 11，菌株类型为CJ2(T) (= ATCC BAA- 779（T) = DSM 15660(T)）。
Strain CJ2(T), capable of growth on naphthalene as a sole carbon and energy source, was T isolated from coal-tar-contaminated freshwater sediment. The Gram reaction of strain CJ2(T) was negative. The cells were non-spore-forming, non-motile cocci (without flagella). The isolate was found to be an aerobic heterotroph capable of utilizing glucose and other simple sugars. Growth was observed between 4 and 25 degreesC (optimum, 20 degreesC) and between pH 6.0 and 9.0 (optimum, pH 7.0-7.5). The G + C content of the genomic DNA was 61.5 mol% and the major quinone was ubiquinone-8. The peptidoglycan of strain CJ2(T) was determined as belonging to type A1-gamma meso-diaminopimelic acid. The major fatty acids of strain CJ2(T) were 16:1omega7c (67.0%), 16:0 (19.6%), 18:1 omega7c (similar to7.9%) and 10:0 3-OH (similar to 2.5%). The polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Mycolic acid and glycolipids could not be detected. Comparative 16S rDNA analysis indicated that strain CJ2(T) is related to the family Comamonadaceae and that the nearest phylogenetic relative was Polaromonas vacuolata 34-p(T) (97.1% similarity). On the basis of the physiological and molecular properties, the naphthalene-degrading isolate was designated Polaromonas naphthalenivorans sp. nov. The type strain is CJ2(T) (= ATCC BAA- 779(T) = DSM 15660(T)).