Cross-linking of initiation factor IF3 to Escherichia coli 30S ribosomal subunit by trans-diamminedichloroplatinum(II): characterization of two cross-linking sites in 16S rRNA; a possible way of functioning for IF3.
Cross-linking of initiation factor IF3 to Escherichia coli 30S ribosomal subunit by trans-diamminedichloroplatinum(II): characterization of two cross-linking sites in 16S rRNA; a possible way of functioning for IF3.
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通过反式二氯二氨铂 (II) 将起始因子 IF3 与大肠杆菌 30S 核糖体亚基交联：16S rRNA 中两个交联位点的表征；
DOI：
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发表时间：
1986
影响因子：
14.9
通讯作者：
B. Ehresmann
中科院分区：
文献类型：
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作者：
C. Ehresmann;H. Moine;M. Mougel;J. Dondon;M. Grunberg‐Manago;J. Ebel;B. Ehresmann
启动因子IF3与反式二氨基氯铂(II)反应，并与大肠杆菌30S核糖体亚基交联。在16S rRNA上明确地确定了两个交联点：一个主要的，在中央结构域的819-859区，和一个次要的，在3‘-末端结构域的1506-1529区。这些序列的特定特征以及它们在30s亚基中的特定位置使我们假设IF3具有作用，这有助于迄今所做的地形和功能观察。
The initiation factor IF3 is platinated with trans-diamminedichloroplatinum(II) and cross-linked to Escherichia coli 30S ribosomal subunit. Two cross-linking sites are unambiguously identified on the 16S rRNA: a major one, in the region 819-859 in the central domain, and a minor one, in the region 1506-1529 in the 3'-terminal domain. Specific features of these sequences together with their particular location within the 30S subunit lead us to postulate a role for IF3, that conciliates topographical and functional observations made so far.