Host Plant Determines the Population Size of an Obligate Symbiont (Buchnera aphidicola) in Aphids
Host Plant Determines the Population Size of an Obligate Symbiont (Buchnera aphidicola) in Aphids
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DOI：
10.1128/aem.04131-15
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发表时间：
2016-02
影响因子：
4.4
通讯作者：
Yuan-Chen Zhang;Wenxue Cao;Le-Rong Zhong;H. Godfray;Xiangdong Liu
中科院分区：
文献类型：
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作者：
Yuan-Chen Zhang;Wenxue Cao;Le-Rong Zhong;H. Godfray;Xiangdong Liu
摘要蚜生布氏菌（Buchnera aphidicola）是一种专性内共生菌，为蚜虫提供多种必需营养物质。虽然蚜虫Buchnera相互作用的了解很多，但寄主植物对Buchnera种群规模的影响仍不清楚。本文采用定量PCR（qPCR）技术研究了寄主植物对棉蚜Buchnera密度的影响。布赫内拉滴度显着较高的群体，已饲养黄瓜超过10年，比在棉花上保持了类似的时间长度的人口。从木槿和西葫芦中收集的蚜虫比从黄瓜和棉花中收集的蚜虫具有更多的Buchnera共生体。蚜虫基因型对布赫内拉种群大小的影响取决于它们所取食的寄主植物。当蚜虫种群保持在黄瓜或棉花转移到新的寄主植物，主机的生存和Buchnera人口规模波动显着的前两代，然后在第三代和以后的几代变得相对稳定。从黄瓜，南瓜，西葫芦，豇豆添加到人工饲料中的寄主植物提取物导致布赫内拉滴度的显着增加，从人口饲养的棉花上的蚜虫，而从棉花和西葫芦的植物提取物导致布赫内拉滴度在黄瓜上饲养的蚜虫下降。棉酚，棉花的次生代谢产物，抑制Buchnera种群从棉花和黄瓜的人口，而葫芦素从葫芦科植物导致更高的密度。总之，结果表明，寄主植物的影响Buchnera人口的过程，这可能会提供表型可塑性在寄主植物使用克隆蚜虫。
ABSTRACT Buchnera aphidicola is an obligate endosymbiont that provides aphids with several essential nutrients. Though much is known about aphid-Buchnera interactions, the effect of the host plant on Buchnera population size remains unclear. Here we used quantitative PCR (qPCR) techniques to explore the effects of the host plant on Buchnera densities in the cotton-melon aphid, Aphis gossypii. Buchnera titers were significantly higher in populations that had been reared on cucumber for over 10 years than in populations maintained on cotton for a similar length of time. Aphids collected in the wild from hibiscus and zucchini harbored more Buchnera symbionts than those collected from cucumber and cotton. The effect of aphid genotype on the population size of Buchnera depended on the host plant upon which they fed. When aphids from populations maintained on cucumber or cotton were transferred to novel host plants, host survival and Buchnera population size fluctuated markedly for the first two generations before becoming relatively stable in the third and later generations. Host plant extracts from cucumber, pumpkin, zucchini, and cowpea added to artificial diets led to a significant increase in Buchnera titers in the aphids from the population reared on cotton, while plant extracts from cotton and zucchini led to a decrease in Buchnera titers in the aphids reared on cucumber. Gossypol, a secondary metabolite from cotton, suppressed Buchnera populations in populations from both cotton and cucumber, while cucurbitacin from cucurbit plants led to higher densities. Together, the results suggest that host plants influence Buchnera population processes and that this may provide phenotypic plasticity in host plant use for clonal aphids.