Molecular cloning of Bral2, a novel brain-specific link protein, and immunohistochemical colocalization with brevican in perineuronal nets
Molecular cloning of Bral2, a novel brain-specific link protein, and immunohistochemical colocalization with brevican in perineuronal nets
复制标题
DOI：
10.1016/s1044-7431(03)00133-7
复制
发表时间：
2003-09-01
影响因子：
3.5
通讯作者：
Oohashi, T
中科院分区：
文献类型：
--
作者：
Bekku, Y;Su, WD;Oohashi, T
透明质酸结合硫酸软骨素蛋白聚糖，称为凝集素，是发育和/或成人大脑中丰富的细胞外基质分子。连接蛋白（LP）也被认为是协调存在于大脑中。我们在这里报告的分子克隆和表达分析的一个新成员的脂多糖：Bral 2，主要在大脑中表达。Bral 2 mRNA的表达首先在P20检测到，并持续到成年，表明其功能的重要性和与成人型lecticans的关联。Bra 12的大量免疫反应性在整个中脑和后脑的几个核中以神经元周围网状模式被发现。原位杂交结果显示Bra 1 2是由这些神经元自身合成的，尤其是小脑皮质的GABA能神经元。有趣的是，通过使用野生型和短蛋白聚糖缺陷小鼠脑的比较免疫组织化学分析，表明Bral 2和短蛋白聚糖在神经元周围网中的共定位和协同重要性。我们的研究结果表明，Bral 2参与细胞外基质的形成，有助于神经元周围网，并促进这些细胞外基质的功能作用的理解。(C)2003 Elsevier Science（美国）。All rights reserved.
The hyaluronan binding chondroitin sulphate proteoglycans, called lecticans, are the abundant extracellular matrix molecules in the developing and/or adult brain. The link proteins (LPs) are also known to be coordinately present in brain. We report here the molecular cloning and expression analysis of a novel member of LPs: Bral2, predominantly expressed in brain. The Bral2 mRNA expression is first detected at P20 and continued through adulthood, suggesting its functional importance and association with adult-type lecticans. The substantial immunoreactivity of Bral2 is found in several nuclei throughout the midbrain and hindbrain in a perineuronal net pattern. In situ hybridization revealed that Bral2 is synthesized by these neurons themselves, especially by the GABAergic neurons in the cerebellar cortex. Interestingly, the colocalization and synergic importance of Bral2 and brevican in the perineuronal nets is indicated by the comparative immunohistochemical analysis using wild-type and brevican-deficient mouse brain. Our results suggest that Bral2 is involved in the formation of extracellular matrix contributing to perineuronal nets and facilitate the understanding of a functional role of these extracellular matrices. (C) 2003 Elsevier Science (USA). All rights reserved.