Altered expression of mucins throughout the colon in ulcerative colitis
Altered expression of mucins throughout the colon in ulcerative colitis
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DOI：
10.1136/gut.40.2.234
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发表时间：
1997-02-01
期刊：
影响因子：
24.5
通讯作者：
Jewell, DP
中科院分区：
文献类型：
--
作者：
Smithson, JE;Campbell, A;Jewell, DP
背景/目的-结肠上皮生物学的区域差异可能决定溃疡性结肠炎的累及程度，本研究中使用了新型单克隆抗体（MAbs）来研究结肠粘膜的区域异质性。方法-使用针对近端结肠和远端结肠中共同抗原的耐受化方法产生的MAbs用于免疫过氧化物酶染色、比较组织化学、免疫印迹、结果结肠特异性单克隆抗体5 F1（IgG（3））和6 G4（IgM）在正常远端结肠中染色杯状细胞内容物，而在近端结肠中染色明显减少（p
Background/Aims-Regional differences in the biology of the colonic epithelium may determine the extent of involvement by ulcerative colitis, Novel monoclonal antibodies (MAbs) were used in this study to investigate regional heterogeneity in the colonic mucosa.Methods-MAbs generated using a method of tolerisation against common antigens in the proximal colon and distal colon were used for immunoperoxidase staining, comparative histochemistry, immunoblotting, and slot-blot analysis.Results-The colon specific MAbs 5F1 (IgG(3)) and 6G4 (IgM) stained goblet cell contents throughout the normal distal colon but staining was markedly reduced in the proximal colon (p