Assessment of the redox profile and oxidative DNA damage (8-OHdG) in squamous cell carcinoma of head and neck
Assessment of the redox profile and oxidative DNA damage (8-OHdG) in squamous cell carcinoma of head and neck
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DOI：
10.4103/0973-1482.98980
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发表时间：
2012-04-01
影响因子：
1.3
通讯作者：
Khandelwal, Shashi
中科院分区：
文献类型：
--
作者：
Kumar, Anil;Pant, Mohan C.;Khandelwal, Shashi
背景：在发展中国家，特别是南亚，各种形式的烟草及其制品消费习惯日益增加。已知这些会产生强烈的自由基环境，当自由基压倒抗氧化系统时，它们可能导致细胞成分的退化和突变。目的：本研究的目的是评估氧化应激决定因素的水平，这可能是头颈癌发展的关键因素之一。材料与方法：本研究包括100例同意SCCHN患者和90名匹配的健康对照，我们评估了总抗氧化能力（TAC），谷胱甘肽（GSH），自由基（RNS，ROS）和氧化DNA加合物（8-OHdG）。结果如下：我们观察到活性氧（ROS，3.0倍）和活性氮（RNS，1.7倍）的大幅上升，同时观察到TAC（1.2倍）和GSH（1.7倍）的显著降低。8-OHdG水平也被发现在患者中显著高于对照组（P <0.05）。血ROS与GSH呈负相关（P <0.01），RNS与DNA损伤呈正相关（P <0.01）。结论：我们目前的研究结果表明，显着的氧化还原不平衡的癌症患者表明他们在SCCHN的发展至关重要。8-OHdG可能是评价SCCHN危险性的潜在生物标志物。为了开发SCCHN预防的新方法，需要对氧化应激和DNA损伤的分子机制进行详细的研究和更好的理解。
Background: In developing countries especially in south Asia, there are growing habits of consumption of tobacco and its products in various forms. These are known to generate a strong free radical environment and when the free radicals overwhelm the antioxidant system, they may lead to degeneration of cellular components and mutations. Aim: The aim of this study is to assess the levels of oxidative stress determinants, which may be one of the critical factors in head and neck cancer development. Materials and Methods: This study included 100 consenting SCCHN patients and 90 matched healthy controls and we assessed the total antioxidant capacity (TAC), glutathione (GSH), free radicals (RNS, ROS) and oxidative DNA adduct (8-OHdG). Results: We observed a substantial rise in reactive oxygen species (ROS, 3.0-fold) and reactive nitrogen species (RNS, 1.7-fold), together with significant lowering in TAC ( 1.2-fold) and GSH ( 1.7-fold) was observed. The 8-OHdG levels were also found to be significantly (P < 0.05) higher in patients in comparison to controls. Pearsons correlation between blood ROS and GSH were found to be negatively correlated -0.38 (P < 0.01) and RNS and DNA damage positively correlated 0.44 (P < 0.01). Conclusion: Our present results demonstrate significant Redox imbalance in cancer patients suggesting their paramount importance in the development of SCCHN. The 8-OHdG could be the potential biomarker for evaluating risk of SCCHN. To develop new approaches of SCCHN prevention, there is a need of detailed study and better understanding of the molecular mechanisms underlying oxidative stress and DNA damage.